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Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCMV-AID-PcrA-UGI
Plasmid#221220PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.DepositorInsertAID-PcrA-UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.TBG.PI.eGFP.WPRE.bGH
Plasmid#105535PurposeAAV expression of EGFP from TBG promoterDepositorHas ServiceAAV8InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterTBGAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
mPiezo1-IRES-eGFP
Plasmid#80925Purposeexpresses mouse Piezo1 with GFPDepositorInsertmouse Piezo1 CDS (Piezo1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP720-N1
Plasmid#136560PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720DepositorInsertmiRFP720
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28sumo-hRig-I-Full-length
Plasmid#210827Purposeprotein expression in ecoliDepositorInserthRIG-I (DDX58) Full length protein (RIGI Human)
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ub-G76V-GFP
Plasmid#11941DepositorInsertUb-G76V-GFP
UseTagsExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …PromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NEmut
Plasmid#123310PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE control sensor GRAB_NEmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-HP1alpha
Plasmid#17652DepositorInsertHP1 alpha (CBX5 Human)
UseTagsGFP and HisExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgAAVS1
Plasmid#192477PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a controlDepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJ806
Plasmid#39312DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationPromoterT7Available sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
CD44S pBabe-puro
Plasmid#19127DepositorInsertCD44 standard (CD44 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_pGK_mCherry
Plasmid#79124PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HIS2-Vps20FL
Plasmid#21490DepositorInsertVPS20 (VPS20 Budding Yeast)
UseTags6x His and MBPExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1
Plasmid#83842PurposeFluorescent probe for S/G2 cell cycle transition and G2/M transitionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianMutationPromoterEF1alpha and IRESAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic PancACe
Plasmid#215708PurposeExpresses acetyl-CoA biosensor in the cytoplasmDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3-SYFP2
Plasmid#189764PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet3-SYFP2
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_hNMT_Nef
Plasmid#66078PurposeSingle vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertsUseTagsExpressionBacterialMutationDeletion of first 80 amino acidsPromoterT7-lacOAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG104 FAST
Plasmid#130720PurposeExpresses FAST (also called YFAST) in mammalian cellsDepositorInsertFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSmartBAC-S
Plasmid#107268PurposeBroad host range bacterial artificial chromosome vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8m-WPRE
Plasmid#179255PurposeAAV-mediated expression of GCaMP8m under the CAG promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8m
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-EGFP
Plasmid#27082PurposeIntegration-free (episomal) expression of EGFPDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHM6-alphasynuclein-A53T
Plasmid#40825DepositorInsertSNCA (SNCA Human)
UseTags6x His and HAExpressionMammalianMutationA53TPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCherry- SEC61B
Plasmid#121160PurposeMammalian expression of mCherry tagged SEC61B, used as a general ER markerDepositorInsertSEC61B (SEC61B )
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCherry
Plasmid#79130PurposeLentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherryDepositorInserttBFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAF0689 Ef1a-Pa01_attB-BFP acceptor
Plasmid#193472PurposeAcceptor plasmid for 3 plasmid recombination assayDepositorInsertPa01_attB
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
plRL117
Plasmid#163635PurposeOptimized M. smegmatis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF2956
Plasmid#144432PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHNF4A (HNF4A Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only