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Plasmid#27290DepositorInsertintegrin alpha V (ITGAV Human)
UseTagsExpressionMammalianMutationIsoform 2PromoterAvailable sinceMarch 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
TEVSH
Plasmid#125194PurposeHis-tagged TEV protease with solubility increasing mutationsDepositorInsertTEV_SH
UseTagsV5 His-tagExpressionBacterialMutationPromoterT7lacAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCasPA
Plasmid#113347PurposeBacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-IDH1-R132H-FLAG
Plasmid#66803PurposeTet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vectorDepositorInsertIDH1 (IDH1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationR132HPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-3XmCherry
Plasmid#64108PurposeExpression of Sp dCas9-3XmCherry in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-PSMD7
Plasmid#22558DepositorInsertPSMD7 (PSMD7 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNurr1-NE
Plasmid#102363PurposeOverexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cellsDepositorInsertNR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
UseTagsNEExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP703-N1
Plasmid#79988PurposeMonomeric near-infrared fluorescent protein miRFP703 in mammalian plasmidDepositorInsertmiRFP703
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-EGFP-WPRE-pA
Plasmid#37091PurposeExpresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertd1eGFP
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT281
Plasmid#122611Purposeexpress evoAPOBEC1-BE4max in mammalian cellsDepositorInsertevoAPOBEC1-BE4max
UseTagsExpressionMammalianMutationE4K H109N H122L D124N R154H A165S P201S F205SPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
FokI-dCas9
Plasmid#52970PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cellsDepositorInsertFok1 fused to dCas9
UseCRISPRTagsFok1 nuclease (homodimeric wildtype) and NLSExpressionMammalianMutationD10A and H840A in Cas9PromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucSM EGFP
Plasmid#90171PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLS-SceI
Plasmid#137725PurposeLentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-MT1-MMP-eGFP
Plasmid#89819PurposeExpress in MT1-MMP mammalian cellsDepositorInsertMT1-MMP (MMP14 Human)
UseRetroviralTagseGFPExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1
Plasmid#83842PurposeFluorescent probe for S/G2 cell cycle transition and G2/M transitionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP2-96-merTetO-EFS-BLaR
Plasmid#118713PurposeContains an optimized 96-mer TetO repeat for imaging of desired lociDepositorInsertsBlasticidin resistance gene
EFS-NS promoter
UseTagsExpressionMammalianMutationPromoterEFS-NSAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-MA4-GFP
Plasmid#114380PurposeDoxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.DepositorInsertMLL-AF4 fusion gene (AFF1 Human)
UseTagsGFPExpressionMammalianMutationPromoterTREAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K(AAG)20-P2A-RFP
Plasmid#105689PurposeDouble fluorescent stalling reporter K(AAG)20DepositorInsertsP2A-3xFLAG-VHPbeta
K(AAG)20
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP16
Plasmid#22595DepositorInsertUSP16 (USP16 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
wtBxb1
Plasmid#222337PurposePlasmid for expressing wild type Bxb1 in the mammalian cellsDepositorInsertCodon-optimized wild type Bxb1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only