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Plasmid#156465PurposeBacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tagDepositorInsertNsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free GFP-NES
Plasmid#182491PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
GFP-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp4
Plasmid#156474PurposeBacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tagDepositorInsertNsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp8
Plasmid#156477PurposeBacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tagDepositorInsertNsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43
Plasmid#134574PurposeExpress human DDX43 protein (WT, full-length) in E. coliDepositorInsertDDX43 (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length WTPromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp7
Plasmid#156476PurposeBacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tagDepositorInsertNsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp10
Plasmid#156479PurposeBacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tagDepositorInsertNsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3a_Ub1
Plasmid#156467PurposeBacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tagDepositorInsertNsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3b_ADRP
Plasmid#156468PurposeBacterial expression of Sars-CoV2 Nsp3b_ADRP protein with His-tag and GST-tagDepositorInsertNsp3b_ADRP (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-K292A
Plasmid#134575PurposeExpress human DDX43 mutant (K292A, ATPase dead) in E. coliDepositorInsertDDX43-K292A (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (K292A)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf10
Plasmid#156486PurposeBacterial expression of Sars-CoV2 Orf10 protein with His-tag and GST-tagDepositorInsertOrf10 (ORF10 Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ:mTREK-1_cryst
Plasmid#133269PurposeP. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFPDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseTagsSNS- 3C_cleavage_site-TAAA-GFPExpressionYeastMutationaa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…PromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-Ost1-alphaf(I)-Btl2
Plasmid#117667PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-alphaf(I)-Btl2
UseTagsExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP(N-glyc)-Tac-TC
Plasmid#162496PurposeExpresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.DepositorInsertpartial length Tac (TMD and cytosolic tail) (IL2RA Human)
UseTagsGFPExpressionMammalianMutationTac is in partial length with its TMD and cysotol…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3c_SUD-N
Plasmid#156469PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-N protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-N (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3c_SUD-M
Plasmid#156470PurposeBacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tagDepositorInsertNsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMMDS 2 colors
Plasmid#206265PurposeExpression of 2 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)DepositorInsertGST mTagBFP1 (Synthetic), Ctnnb1 (M. musculus) (Ctnnb1 )
UseCre-donorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MultiMate-HITI-2c ACTB
Plasmid#206267PurposeAll in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.DepositorInsertSpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
UseCRISPR; Recombinant baculovirus productionTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MultiMate-PE3 HEK3
Plasmid#206273PurposeVector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.DepositorInsertPE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
UseCRISPR; Recombinant baculovirus productionTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MultiMate-PE2 HEK3
Plasmid#206274PurposeVector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.DepositorInsertPE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
UseCRISPR; Recombinant baculovirus production, cre-a…TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only