GFP(N-glyc)-Tac-TC
(Plasmid
#162496)
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PurposeExpresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162496 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4770
- Total vector size (bp) 4866
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Modifications to backboneThe signal peptide of Tac was inserted before GFP tag
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepartial length Tac (TMD and cytosolic tail)
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Alt nameIL2 receptor alpha subunit
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SpeciesH. sapiens (human)
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Insert Size (bp)96
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MutationTac is in partial length with its TMD and cysotolic tail. The GFP tag has one N-glycosylation site.
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GenBank IDNM_000417.3
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Entrez GeneIL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EGFP-CF
- 3′ sequencing primer SV40-PArev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP(N-glyc)-Tac-TC was a gift from Lei Lu (Addgene plasmid # 162496 ; http://n2t.net/addgene:162496 ; RRID:Addgene_162496) -
For your References section:
Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. Sun X, Tie HC, Chen B, Lu L. J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. 10.1074/jbc.RA120.014476 PubMed 32826314