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pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3

Plasmid

#112008

Purpose

For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Insert

axon-GCaMP6s-P2A-mRuby3

Use

AAV and Cre/Lox

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-FKBP-LCa

Plasmid

#59353

Purpose

Expresses human clathrin light chain A tagged with GFP & FKBP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Insert

Clathrin Light Chain A (CLTA Human)

Use

Tags

EGFP and FKBP

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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Fyn-SH3_5WT_S25C

Plasmid

#118824

Purpose

Bacterial expression of the SH3 domain of human Fyn

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

FYN (FYN Human)

Use

Tags

6-His-TEV tag

Expression

Bacterial

Mutation

S25C; See depositor comments below.

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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Pseudojanin (PJ)

Plasmid

#37999

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1
INPP5E (INPP5E Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pEB1deltaC-mCherry

Plasmid

#80603

Purpose

Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

EB1 (MAPRE1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Truncated C-terminus; EB1 coding sequence ends at…

Promoter

CMV

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6-DCX-EGFP

Plasmid

#80599

Purpose

pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

Doublecortin (DCX Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pWPXL-c-Myc

Plasmid

#36980

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

c-Myc (Myc Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pWPI-N3ICD

Plasmid

#185524

Purpose

Constitutive overexpression of human NOTCH3 intracellular domain

Depositor

Eric Shusta, Sean Palecek

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH3 intracellular domain (NOTCH3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEvol-pAzFRS.1.t1

Plasmid

#73547

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.1.t1
pAzFRS.1.t1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Jason Peters, Stanley Qi, Carol Gross

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-actin

Plasmid

#118378

Purpose

Gateway compatible donor vector with WT actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pD21-hgAPOAI

Plasmid

#124962

Purpose

Express ApoAI in the liver

Depositor

Kazuhiro Oka

Article

Belalcazar et al Circulation. 2003 Jun 3;107(21):2726-32.

Insert

human APOAI gene (LPA Human)

Use

Adenoviral

Tags

Expression

Mutation

Promoter

human APOAI

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Akt-FoxO3a-KTR-EGFP

Plasmid

#125131

Purpose

Fluorescent reporter for Akt activity

Depositor

Kazuhiro Aoki

Article

Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

Insert

FOXO2 (FOXO3 Human)

Use

Tags

Enhanced green fluorescent protein (EGFP)

Expression

Mammalian

Mutation

FoxO3A 1-402 amino acid

Promoter

CAG promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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Actin-mRFP-PAGFP

Plasmid

#62382

Purpose

Expresses beta actin with both a PAGFP and mRFP tag.

Depositor

Guillaume Charras, Tim Mitchison

Article

Kueh et al J Cell Biol. 2008 Jul 28;182(2):341-53.

Insert

beta actin (ACTB Human)

Use

Tags

PAGFP and mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pJM483

Plasmid

#52987

Purpose

A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Lactaherin (Mfge8 Mouse)

Use

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Mutation

deleted native signal sequence, deleted C-termina…

Promoter

hsp-16.41

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-FAM208A ts2

Plasmid

#115872

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-FAM208A ts3

Plasmid

#115873

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-CHC17KDP

Plasmid

#59799

Purpose

Expresses knockdown-proof human CHC17 tagged with GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

CHC17 (CLTC Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Silent mutations in amino acids 61-65 to confer s…

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-FAM208A ts1

Plasmid

#115871

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pACE-K2P10.1-StrepTagII

Plasmid

#191475

Purpose

Expresses K2P10.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Article

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P10.1 (KCNK10, TREK-2 Human)

Use

Tags

Expression

Insect

Mutation

N144Q (uniprot P57789-1)

Promoter

polH

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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