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Showing: 2381 - 2400 of 11778 results

  1. pPICZ:mTREK-1_cryst

    Plasmid
    #133269
    Purpose
    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP
    Depositor
    Dan Minor
    Article
    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d
    Insert
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    Use
    Tags
    SNS- 3C_cleavage_site-TAAA-GFP
    Expression
    Yeast
    Mutation
    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…
    Promoter
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    133269-plasmid-map-sequence-id-291942

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  3. GFP(N-glyc)-Tac-TC

    Plasmid
    #162496
    Purpose
    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its TMD and cysotol…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162496-plasmid-map-sequence-id-323476

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  8. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

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  9. MultiMate-PE3 HEK3

    Plasmid
    #206273
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206273-plasmid-map-sequence-id-406645

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  10. MultiMate-PE2 HEK3

    Plasmid
    #206274
    Purpose
    Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE2 HEK3 prime editing. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP
    Use
    CRISPR; Recombinant baculovirus production, cre-a…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206274-plasmid-map-sequence-id-406650

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  12. anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag

    Plasmid
    #204426
    Purpose
    Mammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.
    Depositor
    Jeff Lichtman
    Article
    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d
    Insert
    Recombinant mouse scFv targeting NPY/Neuropeptide Y (Homo sapiens)
    Use
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204426-plasmid-map-sequence-id-406621

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  13. anti-GFAP R416WT scFv [N206B/9] with sortase tag

    Plasmid
    #204420
    Purpose
    Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9.
    Depositor
    Jeff Lichtman
    Article
    Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d
    Insert
    Recombinant mouse scFv targeting GFAP R416WT (Homo sapiens)
    Use
    Tags
    6xHis tag and Sortase tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204420-plasmid-map-sequence-id-406611

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  14. Coexpressed Free NES

    Plasmid
    #182482
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182482-plasmid-map-sequence-id-360445

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  15. Coexpressed Free ClLIS1 (M)

    Plasmid
    #182484
    Purpose
    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS(M)
    LS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10 and GAL7
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182484-plasmid-map-sequence-id-360440

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  16. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid
    #182887
    Purpose
    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    silet mutations in aa 197 - 203 to confer resista…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182887-plasmid-map-sequence-id-367932

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  17. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

    Plasmid
    #182885
    Purpose
    Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    stop codon introduced 2 amino acids downstream of…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182885-plasmid-map-sequence-id-367930

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  19. pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

    Plasmid
    #164945
    Purpose
    Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    KCNJ13 (KCNJ13 Human)
    Use
    Tol2 transposon destination vector
    Tags
    IRES-EGFP
    Expression
    Mutation
    Stop codon deleted
    Promoter
    actinb (actb2) promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164945-plasmid-map-sequence-id-325850

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  20. pDestTol2-actinb-kcnj13-Q153H-EGFP

    Plasmid
    #164943
    Purpose
    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I
    Depositor
    GuangJun Zhang
    Article
    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi
    Insert
    kcnj13 (kcnj13 Zebrafish)
    Use
    Tol2 transposon destination vector
    Tags
    EGFP
    Expression
    Mutation
    Stop codon deleted; Q153H; M154I
    Promoter
    actinb (actb2) promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164943-plasmid-map-sequence-id-325844

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Showing: 2381 - 2400 of 11778 results