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Plasmid#206281PurposeENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMVDepositorInsertmAG-hGeminin (GMNN Human)
UseMultimate/gateway entr 1TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES
Plasmid#182432PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-FPPS-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES
Plasmid#182433PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-FPPS
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (M)
Plasmid#182435PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (G)
Plasmid#182477PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free deadFPPS-NES
Plasmid#182492PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.DepositorInsertsFPPS
deadFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NES-YFP-CCTc
Plasmid#184328PurposeMammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.2)DepositorInsertCCTc (CACNA1C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HsSyx3
Plasmid#179287PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + mature maltose binding protein + TEV prote…ExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HsSyx-375-792
Plasmid#179291PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + mature maltose binding protein + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + mature maltose binding protein + TEV prote…ExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-NT-G46D
Plasmid#134588PurposeExpress human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coliDepositorInsertDDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (G154D)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-FL-G84D
Plasmid#134577PurposeExpress human DDX43 mutant (full-length with G84D mutation) in E. coliDepositorInsertDDX43-G84D (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (G84D)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Tomato
Plasmid#214403PurposeExpresses human SOX10, OLIG2, NKX6.2 and tdTomatoDepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTO_CHMP3_LAP
Plasmid#101849PurposeTet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorInsertCharged Multivesicular Body Protein 3 (CHMP3 Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUltraHot-mCherry-BLM-WT
Plasmid#206966Purposeexpressing the protein BLM fused to mCherry in human cellsDepositorInsertBloom Syndrome RecQ Like Helicase (BLM Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUltraHot-mCherry-DHX36-iso1-WT
Plasmid#206964Purposeexpressing the protein DHX36 fused to mCherry in human cellsDepositorInsertDEAH-Box Helicase 36 (DHX36 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SF-SON IRES Puro
Plasmid#214402PurposeExpresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300 (D1399Y)
Plasmid#191763PurposeExpression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)DepositorInsertFRB-EGFP-p300 core (D1399Y) (EP300 Human)
UseTagsEGFP (N-terminal of p300 core (D1399Y)) and FRB (…ExpressionBacterial and MammalianMutationcatalytic D1399Y mutation in p300PromoterEF1alphaAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA
Plasmid#85756PurposeAll in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).DepositorInsertsCsy4-T2A-SpRFN-P2A-GFP
gRNA
UseCRISPRTagsCsy4, Csy4 recognition sequence, FokI fusion via …ExpressionMammalianMutationD10A, H840APromoterCMV and U6Available sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMS-dsRed2-C1824U LMNA-GFP minigene
Plasmid#128231PurposeC1824U mutation Lamin A splicing reporterDepositorInsertlamin A (LMNA Human)
UseTagsGFP and SV40-driven dsRed2 to identify transfecte…ExpressionMammalianMutationC1824UPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)
Plasmid#169189PurposeBaculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cellsDepositorInsert3xFlag-His6-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
UseTags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only