pUltraHot-mCherry-DHX36-iso1-WT
(Plasmid #206964)

• Purpose: expressing the protein DHX36 fused to mCherry in human cells
• Depositing Lab: Eran Hornstein
• Publication: Danino et al Nucleic Acids Res. 2023 Jul 28:gkad613. doi: 10.1093/nar/gkad613.

Backbone

• Vector backbone: pUltraHot
• Backbone size w/o insert (bp): 7475
• Total vector size (bp): 11204
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DEAH-Box Helicase 36
• Alt name: DHX36
• Alt name: RHAU
• Species: H. sapiens (human)
• Insert Size (bp): 3021
• GenBank ID: NM_020865.3
• Entrez Gene: DHX36 (a.k.a. DDX36, G4R1, MLEL1, RHAU)
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: tccaccggcggcatggacgagctgtacaagAGTTATGACTACCATCAGAACTGG
• 3′ sequencing primer: ctccggaacgaattctgatcacacgctagcTCAGCTGTAATATCCATCCTGGAA

Resource Information

• Supplemental Documents:
  • pultra-hot-mcherry-dhx36-iso1-wt-yehuda.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry