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iRFP-C1

Plasmid

#54786

Purpose

Localization: C1 Cloning Vector, Excitation: 690, Emission: 713

Depositor

Michael Davidson, Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Type

Empty backbone

Use

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SuExp His-Myc-mPLD4

Plasmid

#201185

Purpose

Protein expression plasmid for recombinant His-Myc tag mouse PLD4

Depositor

David Nemazee

Article

Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

Insert

PLD4 (Pld4, Pld4, Pld4 Mouse)

Use

Tags

His-Myc, Factor Xa cleavable

Expression

Mammalian

Mutation

cytosolic and transmembrane domain truncated; add…

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPSU30

Plasmid

#173830

Purpose

Expresses 30 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPACS100BCBP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPSU10

Plasmid

#173827

Purpose

Expresses 10 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-spRYCas9-ABE-UMax

Plasmid

#222139

Purpose

Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

TadA-nSpRYCas9

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pBT376

Plasmid

#125617

Purpose

Expresses BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC T18A,S19A

Plasmid

#35681

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T18A and S19A

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

Plasmid

#163543

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-nCas9-UdgX

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)

Promoter

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pH-ePPE

Plasmid

#183097

Purpose

For plant prime editing in rice plants

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpCas9

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-mTubb3

Plasmid

#87116

Purpose

AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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PDHA1-N-10

Plasmid

#58195

Purpose

Localization: Mitochondria, Excitation: N/A, Emission: N/A

Depositor

Michael Davidson

Insert

PDHA1 (PDHA1 Human)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCMV-lyso-pHluorin

Plasmid

#70113

Purpose

Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHluorin (Cd63 Aequorea victoria, Mouse)

Use

Tags

HA-Tag and pHluorin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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mTAGBFP2 Peroxisome visual barcode

Plasmid

#158668

Purpose

tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mTAGBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-Uricase

Plasmid

#182401

Purpose

Bacterial expression of Vesicle Nucleating peptide-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-uricase

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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SFFV-IKZF3-NTERM-GFP

Plasmid

#185764

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

William Sellers, Alessandra Ianari

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Expression

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-GATA4

Plasmid

#192896

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

GATA4 (GATA4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Codon-optimized

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLuc.mKO2

Plasmid

#85224

Purpose

shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

Firefly Luciferase

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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SFFV-SMASH-NTERM-GFP

Plasmid

#185761

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

William Sellers, Alessandra Ianari

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Expression

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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