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Plasmid#107057PurposeExpression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationK785RPromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1-TelR15-sfGFP
Plasmid#49645PurposeTALEGreen-Telomere for In Vitro TranslationDepositorInsertTelR15
UseIn vitro translationTagssfGFPExpressionMutationPromoterT7Available sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-rnc-sfGFP
Plasmid#51560PurposeGene encoding E. coli RNaseIII cloned as a N terminal translational fussion with superfolder GFP. Expression driven by arabinose (backbone is pBAD24).DepositorInsertrnc (rnc E. coli)
UseTagsGLESTCRHASLAVLADERRFSA and superfolder GFPExpressionBacterialMutationContains additonal aa at the end of the sfGFP in …PromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
RHPMP192 (pLifeAct sfGFP)
Plasmid#88843PurposeLocalization by Fluorescence microscopy of lifeAct fused to super folder GFPDepositorInsertLifeAct
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(cyto).cpSFGFP.HaloTag
Plasmid#209662PurposeNon-responsive controlDepositorInsertcpSFGFP.HaloTag
UseAAVTagsExpressionMutationPromoterGFAPAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT2 NCmut-msfGFP
Plasmid#180320Purposemammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFPDepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT2 F20D, V27DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.4-sfGFP
Plasmid#149666PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP trigger
Plasmid#107359PurposeTrigger sequence for pNP1 sfGFP toehold switchDepositorInserttoehold trigger 7
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(8G)-mCherry
Plasmid#206950PurposeMammalian expression of heterobifunctional SasG linkers (8G)DepositorInsertsfGFP-SaSasG (8G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-mCherry-sfGFP
Plasmid#213860PurposeFor Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertmCherry-sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterCaMV 35SAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
prCAG-RBS"m1"-sfGFP
Plasmid#191400PurposeExpression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)DepositorInsert47xCAG RBS"m1"-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLtet0-1Available sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_minSV40-sfGFP-CMV_dsRed
Plasmid#117400PurposesfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
RGD-sfGFP-I27-CC
Plasmid#85392PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labelingDepositorInsertRGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-3xSV40pA-F-td-sfGFP
Plasmid#133570PurposeCAG tdGFP FLP/FRT reporter piggyBac transposon (3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-DQNAT
Plasmid#198210PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Double UP sfGFP to mScarlet
Plasmid#120261PurposeNatively produces sfGFP, in the presence of Cre will be recombined to produce mScarlet.DepositorInsertsfGFP/mScarletP2A-mcs
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterCAGAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2-sfGFP
Plasmid#145173PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v6-sfGFP
Plasmid#145177PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 6DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-sfGFP
Plasmid#114598Purpose"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterPBenAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-sfGFP
Plasmid#145172PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only