pT7CFE1-TelR15-sfGFP
(Plasmid
#49645)
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PurposeTALEGreen-Telomere for In Vitro Translation
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 49645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT7CFE1
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Backbone manufacturerPierce
- Backbone size w/o insert (bp) 3600
- Total vector size (bp) 6300
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Vector typeIn vitro Translation
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTelR15
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SpeciesSynthetic
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Insert Size (bp)2000
- Promoter T7
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Tag
/ Fusion Protein
- sfGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SbfI (not destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7CFE1-TelR15-sfGFP was a gift from Thoru Pederson (Addgene plasmid # 49645 ; http://n2t.net/addgene:49645 ; RRID:Addgene_49645) -
For your References section:
Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors. Ma H, Reyes-Gutierrez P, Pederson T. Proc Natl Acad Sci U S A. 2013 Dec 9. 10.1073/pnas.1319097110 PubMed 24324157