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mCerulean3-31aa-Venus-pEGFP-C3

Plasmid

#166754

Purpose

For expression of mCerulean3 linked to Venus by 31 amino acid linker

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3-31aa-Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST H2B-mCerulean3

Plasmid

#90234

Purpose

Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxON-pre-mGRASP-mCerulean

Plasmid

#51903

Purpose

JxOn-pre

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

CAG

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204971

Purpose

Soma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Article

Oppermann et al eLife 2023 (Reviewed Preprint)

Insert

raACR

Use

AAV

Tags

mCerulean3

Expression

Mutation

Promoter

CaMKII

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pUSE-Src-YF-UniRapR-mCerulean-myc

Plasmid

#45381

Depositor

Klaus Hahn

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src-YF-UniRapR-mCerulean-myc

Use

Tags

Myc, Myr, and mCerulean

Expression

Mammalian

Mutation

changed mSrc Tyrosine 529 to Phenylalanine

Promoter

CMV

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDUET-1-alpha-synuclein-mCerulean3-His6

Plasmid

#110061

Purpose

For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha-synuclein (SNCA Human)

Use

Tags

6 x His and mCerulean3

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxOFF-pre-mGRASP-mCerulean

Plasmid

#51900

Purpose

JxOFF-pre

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS

Plasmid

#162616

Purpose

Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mCerulean

Use

Tags

Expression

Mammalian

Mutation

156-239

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mCerulean LIC cloning vector (6E)

Plasmid

#30128

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pUSE-Src-KD-UniRapR-mCerulean-myc

Plasmid

#45382

Depositor

Klaus Hahn

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src-KD-UniRapR-mCerulean-myc

Use

Tags

mCerulean and myc

Expression

Mammalian

Mutation

mSrc R388D (kinase dead), Y529F

Promoter

CMV

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Ef1a-somBiPOLES-mCerulean-IRES-Puro

Plasmid

#192585

Purpose

lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

Lentiviral

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-mCerulean3-cpVenus[E172]

Plasmid

#125203

Purpose

SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET high control

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

mCerulean3-cpVenus[E172]

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mCerulean-PA-Rac1

Plasmid

#22031

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pME-mCerulean3-TUBB1A (JDW 1367)

Plasmid

#224503

Purpose

Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

mCerulean3-TUBB1A

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME H2B mCerulean (JDW 1150)

Plasmid

#224543

Purpose

A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

H2B-mCerulean

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204970

Purpose

Soma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Article

Oppermann et al eLife 2023 (Reviewed Preprint)

Insert

MsACR1

Use

AAV

Tags

mCerulean3

Expression

Mutation

Promoter

CaMKII

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-cjSTELLA-mCerulean-PuroTK

Plasmid

#141029

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

mCerulean

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry

Plasmid

#34911

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75154

Purpose

Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS-TDP-43

Plasmid

#162617

Purpose

Allows for transcription of mCerulean fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

TARDBP (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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