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Addgene
Showing: 221 - 240 of 4601 results

  4. pLV-mScarlet-I

    Plasmid
    #159172
    Purpose
    To express a bright monomeric red FP for labelling and detection of mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mScarlet-I
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Codons were optimized to Human codon usage
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    159172-plasmid-map-sequence-id-311154

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  5. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid
    #126472
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    Or47a (Or47a Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    126472-plasmid-map-sequence-id-246570

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  6. pDmelOR

    Plasmid
    #126476
    Purpose
    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    DmelOrco (Orco Fly)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens; includes a rec…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    126476-plasmid-map-sequence-id-246523

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  15. pCRISPomyces-2

    Plasmid
    #61737
    Purpose
    Streptomyces expression of codon-optimized Cas9 and custom gRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    gRNA cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL) and rpsL(XC)-BbsI
    Availability
    Academic Institutions and Nonprofits only
    61737-plasmid-map-sequence-id-190162

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  17. FCK-Halo-GFP

    Plasmid
    #14750
    Purpose
    Lentiviral expression of Halo-GFP for neuronal inhibition
    Depositor
    Edward Boyden
    Article
    Han et al PLoS One. 2007 Mar 21;2(3):e299.
    Insert
    Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    N. pharaonis halorhodopsin is mammalian codon-opt…
    Promoter
    CaMKIIa
    Availability
    Academic Institutions and Nonprofits only
    14750-plasmid-map-sequence-id-192940

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Showing: 221 - 240 of 4601 results