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We narrowed to 13,875 results for: LEA

Showing: 221 - 240 of 13875 results

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pKER-mCerulean

Plasmid

#86622

Purpose

Expresses mCerulean from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mCerulean

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pJC20Cerulean3-Cdc8

Plasmid

#99357

Purpose

Bacterial expression of Cerulean3 fused to the 5' of cDNA of the fission yeast tropomyosin, cdc8.

Depositor

Dan Mulvihill

Article

Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

Insert

cdc8 (cdc8 Fission Yeast)

Use

Tags

Cerulean3 and His6-Gly3

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8-cerulean3

Plasmid

#99358

Purpose

Bacterial expression of Cerulean3 fused to the 3' of cDNA of the fission yeast tropomyosin, cdc8.

Depositor

Dan Mulvihill

Article

Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

Insert

cdc8 (cdc8 Fission Yeast)

Use

Tags

Cerulean3 and Gly3-His6

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mCerulean3

Plasmid

#96936

Purpose

Retroviral plasmid containing mCerulean3 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Cerulean 3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A3_self-cleavingCas9

Plasmid

#130281

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAAV_COL4A5_self-cleavingCas9

Plasmid

#130282

Purpose

Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

spCas9

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 bleach.2

Plasmid

#191878

Purpose

Encodes a GFP design, bleach.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.2

Use

Tags

His Tag

Expression

Bacterial

Mutation

V61L T65S V68A S72A V112I Y145M

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 bleach.6

Plasmid

#191879

Purpose

Encodes a GFP design, bleach.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.6

Use

Tags

His Tag

Expression

Bacterial

Mutation

V61L S72C Y145M H181L V224I

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 bleach.1

Plasmid

#191885

Purpose

Encodes a GFP design, bleach.1

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.1

Use

Tags

His Tag

Expression

Bacterial

Mutation

T108L Y145M T167V L220V

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 bleach.3

Plasmid

#191914

Purpose

Encodes a GFP design, bleach.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.3

Use

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145M T167V H181I V224I

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 sf:bleach.3

Plasmid

#191925

Purpose

Encodes a GFP design, sf:bleach.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:bleach.3

Use

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145M T167V H181I V224I

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 sf:bleach.1

Plasmid

#191929

Purpose

Encodes a GFP design, sf:bleach.1

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:bleach.1

Use

Tags

His Tag

Expression

Bacterial

Mutation

T108L Y145M T167V L220V

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRFLIP38-AtLEA4-5

Plasmid

#178189

Purpose

SED1 biosensor for expression in Saccharomyces cerevisiae

Depositor

Jose Dinneny, Cesar Cuevas-Velazquez

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Use

Tags

Expression

Yeast

Mutation

Promoter

pPMA1

Availability

Academic Institutions and Nonprofits only

Enlarge
PEA1-Nuclease

Plasmid

#176528

Purpose

Delivers all prime editing nuclease components in a single plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

GC to CA point mutation in SpCas9 to restore nucl…

Promoter

CbH for Cas9, hU6 for gRNAs

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean(1-10)

Plasmid

#165144

Purpose

Expresses Cerulean(1-10) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

Cerulean(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
MsACR1-pmCerulean3-N1

Plasmid

#204958

Purpose

Expression of channelrhodopsin MsACR1 fused to mCerulean3 in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife 2023 (Reviewed Preprint)

Insert

MsACR1

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV (+enhancer)

Availability

Academic Institutions and Nonprofits only

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raACR-pmCerulean3-N1

Plasmid

#204959

Purpose

Expression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife 2023 (Reviewed Preprint)

Insert

raACR

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV (+enhancer)

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_LEA1_APHAV (pBS0335)

Plasmid

#185180

Purpose

For the mammalian expression of the nematode (Aphelenchus avenae) protein LEA1_APHAV. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

LEA1_APHAV

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_10 (pBS0705)

Plasmid

#185216

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_mutant_14 (pBS0709)

Plasmid

#185217

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_14. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_14

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge