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pLentiPGK Hygro DEST H2B-mCerulean3

Plasmid

#90234

Purpose

Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

Plasmid

#117405

Purpose

SpyCas9 sgRNA 1 targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SpyCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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TPI in pCW57_tTA_Blast

Plasmid

#154209

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE repeats (dox off)

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMA3288

Plasmid

#46885

Purpose

Lentiviral vector encoding Tet-inducible mitochondrially targeted Wild Type human Uracil-N-glycosylase

Depositor

Mikhail Alexeyev

Article

Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

Insert

Uracil-N-glycosylase WT (UNG Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

TRE-Tight

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-9

Plasmid

#208787

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 9 (CASP9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-C91A

Plasmid

#125841

Purpose

express mutant BAP1-C91A

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

C91A

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-V5-KDM3A-mut

Plasmid

#82332

Purpose

lentiviral expression of demethylase-inactive KDM3A H1120G/D1122N

Depositor

Luke Gaughan

Article

Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

Insert

KDM3A (KDM3A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

demethylase-inactive H1120G/D1122N and two silent…

Promoter

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176272

Purpose

Expression of non-targeting sgRNA of CasMINI

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI non-targeting sgRNA (sgLacZ) and BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

Plasmid

#204146

Purpose

Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Inserts

SARS-CoV-2 BA.1 variant spike
zsGreen-T2A-PuR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV and TRE3G

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Rtn4a

Plasmid

#86683

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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plko-shmfn2-mito-GFP-ER tethering

Plasmid

#160509

Purpose

Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mito-GFP-ER tethering

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNTI828

Plasmid

#216636

Purpose

cpTurboID-ERM (V5) ER membrane-localized cpTurboID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol (2024)

Insert

cpTurboID-ERM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI935

Plasmid

#216638

Purpose

cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol (2024)

Insert

cpTurboID-PM

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Plasmid

#163057

Purpose

Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP1-iEER

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti SpBsmBI sgRNA Puro

Plasmid

#62207

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

Depositor

Rene Maehr

Article

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-start

Plasmid

#127168

Purpose

Entry plasmid for two-step guide-barcode cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

N/A

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf10-2xStrep-IRES-Puro

Plasmid

#141394

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf10 (ORF10 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF3034

Plasmid

#144510

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

OTX1 (OTX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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LV-hNG2-GFP

Plasmid

#183910

Purpose

Expression of GFP under the human NG2 promoter

Depositor

Chun-Li Zhang

Article

Tai et al Cell Stem Cell. 2021 May 6;28(5):923-937

Insert

GFP (GFAP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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7TFC

Plasmid

#24307

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-FFluc // SV40-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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