-
Plasmid#16243DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHAExpressionMammalianMutationDominant negative (K179M)PromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM2-Halo-FRB
Plasmid#197056PurposeExpression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-G3BP1-DeltaRBP
Plasmid#135999PurposeG3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminusDepositorInsertG3BP1 (G3BP1 Human)
UseTagsEGFPExpressionMammalianMutationC-term of G3BP1 Deleted for Delta RBPPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCH61 (multiAsCas12a-KRAB piggyBac)
Plasmid#217332PurposepiggyBac expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92392Purposeproduces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTADepositorInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-mPD-L1
Plasmid#121488PurposeExpression of mouse PD-L1 WTDepositorInsertPD-L1
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM4-Halo-FRB
Plasmid#197057PurposeExpression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM7-Halo-FRB
Plasmid#197058PurposeExpression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM22-Halo-FRB
Plasmid#197059PurposeExpression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_FRB-GEM23-Halo
Plasmid#197060PurposeExpression of nanocage I3-01 Halo-FRB-fusion in mammalian cellsDepositorInsertI3-01
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGIPZ-PD-L1-EGFP
Plasmid#120933PurposeExpress PD-L1-EGFP fusion protein in mammalian cellsDepositorInsertPD-L1-EGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tag5Amyc-GSK3b CA
Plasmid#16261DepositorInsertGSK3 beta (GSK3B Human)
UseTagsMycExpressionMammalianMutationConstitutively active: S9APromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-mCherry-P2A-NLSFlpN-nMagHigh
Plasmid#180585PurposePA-FlpDepositorInsertSplit FlpO
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT(dRH) for Split-PE (pEH50)
Plasmid#190106PurposeExpresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE useDepositorInsertMMLV-RT(delta RNAseH)
UseTagsbpNLSExpressionMammalianMutationmutations from RT in PE2 and truncation of RNAse …PromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-G3BP1-CDS
Plasmid#136039PurposeG3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)DepositorInsertG3BP1 (G3BP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-G3BP1-3'UTR
Plasmid#136038PurposeG3BP1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTGTAAGAAATACAGGATT)DepositorInsertG3BP1 (G3BP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEH371 CD63 HA
Plasmid#168220PurposeMammalian expression of tetraspanin CD63 fused to a C-terminal HA tagDepositorInsertCD63 (CD63 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
RhGC(BE)_pGEM
Plasmid#85469PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertrhodopsin-guanylyl cyclase
UseTagsExpressionMammalianMutationPromoterT7 PromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
SQT1665
Plasmid#78744PurposeHuman expression plasmid for LbCpf1-NLS-3xHADepositorInsertmammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCAGAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -