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Showing: 2361 - 2380 of 24268 results

  5. CMV-ER-LAR-GECO1

    Plasmid
    #61244
    Purpose
    Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells
    Depositor
    Robert Campbell
    Article
    Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi
    Insert
    LAR-GECO1
    Use
    Tags
    ER-retention sequence: KDEL and ER-targeting sequ…
    Expression
    Mammalian
    Mutation
    Substitutions relative to R-GECO1: V51W/I113V/N35…
    Promoter
    CMV
    Available since
    March 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61244-plasmid-map-sequence-id-117077

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  9. pTXB1-pA-M.EcoGII

    Plasmid
    #192873
    Purpose
    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578
    Depositor
    Chong Tang
    Article
    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:
    Insert
    pA-M.EcoGII
    Use
    Tags
    3X Flag Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    192873-plasmid-map-sequence-id-380474

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  11. pdCas9::BFP-humanized

    Plasmid
    #44247
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 fused to BFP
    Use
    CRISPR and Retroviral
    Tags
    2xNLS & tagBFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44247-plasmid-map-sequence-id-60248

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  15. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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  20. pAAV hSyn1 YFP-CaRhAC

    Plasmid
    #101721
    Purpose
    Yellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellu
    Depositor
    Thomas Oertner
    Article
    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:
    Insert
    YFP CaRhAC
    Use
    AAV
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn1
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    101721-plasmid-map-sequence-id-201305

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Showing: 2361 - 2380 of 24268 results