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pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Article

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSR657

Plasmid

#65214

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-2 cap

Use

Tags

Expression

Insect

Mutation

Promoter

p10 and polyhedrin

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-Sumo1 WT

Plasmid

#48966

Purpose

Expresses HA-Sumo1 WT in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO1 (SUMO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-Pontin

Plasmid

#51635

Purpose

Mammalian expression of 3xFLAG tagged pontin, N-terminal.

Depositor

Steven Artandi

Article

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

Pontin (RUVBL1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCSCX

Plasmid

#63918

Purpose

Expression of the sucrose utilization phenotype

Depositor

Claudia Vickers

Article

Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

Insert

cscAKB

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-HA

Plasmid

#130910

Purpose

Expresses human cGAS-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pK19pA-MN

Plasmid

#86973

Purpose

ChIC; genomic mapping of chromatin proteins

Depositor

Ulrich Laemmli

Article

Schmid et al Mol Cell. 2004 Oct 8;16(1):147-57.

Insert

protein_A fused to Staphylococcus aureus microccocal nuclease (MNase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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mito-BFP

Plasmid

#49151

Purpose

visualization of mitochondria

Depositor

Gia Voeltz

Article

Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

Insert

mitochondrial targeting signal (COX4 Budding Yeast)

Use

Tags

BFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLARRY-EGFP

Plasmid

#140025

Purpose

Lentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNA

Depositor

Fernando Camargo

Article

Weinreb et al Science. 2020 Jan 23. pii: science.aaw33

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCCL-c-MNDU3-X

Plasmid

#81071

Purpose

Empty lentiviral backbone with the MNDU3 promoter

Depositor

Donald Kohn

Article

Logan et al Hum Gene Ther. 2004 Oct;15(10):976-88.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MNDU3

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV V5-LUC Blast (w567-1)

Plasmid

#21474

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, Blast

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

V5-LUC

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pLX303-ABI1-dCas9

Plasmid

#154474

Purpose

Expresses ABI1 fused to the N terminus of dCas9

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ABI1 (ABI1 Mustard Weed)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Includes ABI1 aa 126-423, D143A

Promoter

CMV

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFB1

Plasmid

#116799

Purpose

Lentiviral expression of TGFB1

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBI-MCS-EGFP

Plasmid

#16542

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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pCGN-MEF2B

Plasmid

#32959

Purpose

For mammalian expression of human MEF2B with an HA tag

Depositor

Ron Prywes

Article

Han et al Mol Cell Biol. 1995 Jun;15(6):2907-15.

Insert

MEF2B (MEF2B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-hPIKfyve

Plasmid

#121148

Purpose

Expresses GFP-tagged human PIKfyve in mammalian cells.

Depositor

Geert van den Bogaart

Article

Baranov et al iScience. 2019 Jan 25;11:160-177. doi: 1

Insert

1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Raichu-RhoA-CR

Plasmid

#40258

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Raichu-RhoA-CR (RHOA Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCH45 (multiAsCas12a-KRAB lenti)

Plasmid

#217330

Purpose

Lentiviral expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mutation

R1226A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Aviv Regev, Joan Brugge

Article

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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