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Showing: 2301 - 2320 of 3328 results

  2. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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  15. pYHY2

    Plasmid
    #216108
    Purpose
    Constitutive expression of secretory BT0294 SP-NanoLuc via BfP1E6-RBS8 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT0294 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    BfP1E6 promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216108-plasmid-map-sequence-id-434369

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  16. pJB06

    Plasmid
    #190480
    Purpose
    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Article
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Inserts
    Cas9
    XylR
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Tags
    Expression
    Mutation
    Promoter
    xylR-driven promoter
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    190480-plasmid-map-sequence-id-374623

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  17. pJB07

    Plasmid
    #190481
    Purpose
    Targeting plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Article
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Inserts
    Upstream & Downstream pyrE deletion region
    gRNA
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190481-plasmid-map-sequence-id-372027

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  18. pMZ4004

    Plasmid
    #204401
    Purpose
    A 3OHC14-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.
    Depositor
    Lauren Andrews
    Article
    Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102
    Inserts
    mCinR
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PftsH and Synthetic promoter PCin0102D
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    204401-plasmid-map-sequence-id-403612

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  20. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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Showing: 2301 - 2320 of 3328 results