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Plasmid#111156PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInserthShh (SHH Human)
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationwtPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
hSERT-N1-pECFP
Plasmid#70106Purposemammalian expression of hSERT with C-terminal CFP fusionDepositorInsertSERT (SLC6A4 Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ1001
Plasmid#204398PurposeA pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRpa0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ3012
Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRhl0L0L12Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE335
Plasmid#153244PurposeM3 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas12j2
Plasmid#173924PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLSDepositorInsertNLS-Cas12j2-NLS
UseCRISPRTagsNLSExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC114
Plasmid#118904Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase siteDepositorInsertNcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with TEV protease, two glycinesExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…ExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL1
Plasmid#197285PurposeShuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195DepositorInsertOrigin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTH
Plasmid#56659PurposeDual-expression vector for P. tricornutumDepositorInsertKanR
UseSynthetic Biology; Diatom expressionTagsExpressionMutationPromoterAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDGE496
Plasmid#153248PurposeM6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE495
Plasmid#153247PurposeM5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE336
Plasmid#153245PurposeM4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE341
Plasmid#153250PurposeM7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRL1124
Plasmid#70690Purposeprovides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.DepositorInsertsavaiM
eco47iiM
ecot22iM
UseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST-KNarK2
Plasmid#44565Purposefor ectopic hypoxia inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDGE662
Plasmid#153260PurposeM6 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE664
Plasmid#153262PurposeM7 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE659
Plasmid#153257PurposeM4 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE661
Plasmid#153259PurposeM5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only