Addgene

We narrowed to 6,225 results for: sms

Showing: 2301 - 2320 of 6225 results

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pCW57.1-Luciferase

Plasmid

#99283

Purpose

All-in-one lentivector with tet-inducible luciferase

Depositor

Stephen Tapscott

Article

Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Promoter

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMS-II-dCas13-APO1-YTH

Plasmid

#204767

Purpose

GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

dCas13-NLS-APO1-YTH-HA

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Ef1alpha

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Use

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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TRE-VEGFA EF1a-rtTA3

Plasmid

#195881

Purpose

Lentiviral construct of doxycycline inducible VEGFA

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a)

Plasmid

#79672

Purpose

Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP (Scn9a jellyfish, Mouse)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1-1482 aa)

Plasmid

#21745

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1-1482) (Mtor Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

amino acids 1-1482

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
myc-mTOR (1750-2549 aa)

Plasmid

#21747

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1750-2549) (Mtor Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

amino acids 1750-2549

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pCMVTAG-NEMO

Plasmid

#11970

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMO (IKBKG Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only

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pTU1-A-SP44-sfGFP

Plasmid

#163757

Purpose

Expresses Streptomyces codon-optimised sfGFP

Depositor

Simon Moore, Paul Freemont

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

SP44

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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His-PIMT

Plasmid

#34852

Depositor

Bruce Downie

Article

Dinkins et al Plant J. 2008 Jul;55(1):1-13.

Insert

PIMT (PCMT1 Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-eNme2-C.NR

Plasmid

#185672

Purpose

Expresses nuclease active eNme2-C.NR in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

eNme2-C.NR nuclease

Use

CRISPR

Tags

Expression

Mammalian

Mutation

eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

Promoter

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTU1-A-SP44-mScarlet-I

Plasmid

#163756

Purpose

Expresses Streptomyces codon-optimised mScarlet-I

Depositor

Simon Moore, Paul Freemont

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

mScarlet-I

Use

Synthetic Biology

Tags

Flag and FlAsH tags

Expression

Bacterial

Mutation

Promoter

SP44

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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TPI in pCW57_tTA_Blast

Plasmid

#154209

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE repeats (dox off)

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δrev

Plasmid

#101348

Purpose

All mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta rev

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-DAXX-mSIM1

Plasmid

#134524

Purpose

Expresses YFP-DAXX fusion protein with a mutation in its 5' SIM

Depositor

Susan Janicki

Article

Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

Insert

DAXX-mSIM1

Use

Tags

YFP

Expression

Mammalian

Mutation

SIM (IIVL) 7-10 aa changed to IGAG

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-D

Plasmid

#38133

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-D (MITF Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pBS hShh (CT#401)

Plasmid

#13996

Depositor

Cliff Tabin

Article

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Shh (SHH Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pLexM-SLC3A2

Plasmid

#197994

Purpose

Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells

Depositor

Simon Newstead

Article

Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

Insert

SLC3A2 (SLC3A2 Human)

Use

Tags

His tag

Expression

Mammalian

Mutation

Please see depositor comments below

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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