Addgene

Showing: 2301 - 2320 of 3064 results

Results per page:

Sort results by:

PH-Btk-GFP

Plasmid

#51463

Purpose

Is a biosensor for PIP3

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

BTK (aa 1–177) (BTK Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-AcGFP1-ORP9-PH

Plasmid

#214266

Purpose

Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

AcGFP1-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Mammalian expression: Z-lock cofilin dark state mutant

Plasmid

#141136

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dTomato-ORP9-PH

Plasmid

#214268

Purpose

Mammalian expression of red fluorescent PI4P reporter dTomato-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

dTomato-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pALB-GFP

Plasmid

#55759

Purpose

Fluorescent marker for hepatocytic lineage

Depositor

Snorri Thorgeirsson

Article

Heo et al Hepatology. 2006 Dec;44(6):1478-86.

Insert

albumin enhancer/promoter (Alb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Mouse Albumin Enhancer/Promoter

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mTagBFP2-ORP9-PH

Plasmid

#214267

Purpose

Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
VPS13A^Halo

Plasmid

#118759

Purpose

VPS13A internally tagged with Halo at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Use

Tags

Halo tag inserted at position 1372

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mouse vinculin full length (889)

Plasmid

#67935

Purpose

Mammalian expression of mouse vinculin with GFP fusion

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

Vinculin (Vcl Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L159P in vinculin

Promoter

CMV

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
tdTomato-KIF1A

Plasmid

#102850

Purpose

Overexpresses KIF1A and cell fill TdTomato

Depositor

Erik Dent

Article

McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

Insert

KIF1A (Kif1a Rat)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
G-CaMP3

Plasmid

#22692

Purpose

a single-wavelength GCaMP2-based genetically encoded calcium indicator

Depositor

Loren Looger

Article

Tian et al Nat Methods. 2009 Dec;6(12):875-81.

Insert

G-CaMP3

Use

Tags

Expression

Mammalian

Mutation

N363D mutation (increased fluorescence)

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-NFAT1(4-460)-GFP

Plasmid

#11107

Purpose

Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

Depositor

Anjana Rao

Article

Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

Insert

NFAT1 (Nfatc2 Mouse)

Use

Tags

3x HA and GFP

Expression

Mammalian

Mutation

Contains amino acids 4-460 of NFAT1; L78P compare…

Promoter

CMV

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Alpha 5 integrin-GFP

Plasmid

#15238

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

alpha 5 integrin (ITGA5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-epsin(144-575)-GFP

Plasmid

#100733

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

epn1 (Epn1 Mouse)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

clathrin binding middle part aa 144-575

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LifeAct-GFP-Fis1

Plasmid

#182576

Purpose

Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

LifeAct-GFP-Fis1

Use

Tags

Fis1 and LifeAct

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-hPMCA2w/b

Plasmid

#47586

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b (ATP2B2 Human)

Use

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
SspB-mCherry-DHPH-TIAM1

Plasmid

#176104

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIBN(deltaNLS)-pmGFP

Plasmid

#26867

Purpose

Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

CMV

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ERK1

Plasmid

#14747

Depositor

Rony Seger

Article

Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

Insert

ERK1 (MAPK3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
ankG-mCherry

Plasmid

#42566

Depositor

Benedicte Dargent

Article

Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

ankyrin G (Ank3 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-2PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge