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pMH-06

Plasmid

#158434

Purpose

pBAD33 vector encoding MotA, Dendra2-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Use

Tags

Dendra2

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-03

Plasmid

#158433

Purpose

pBAD33 vector encoding MotA, yPET-MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Use

Tags

yPET

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA

Plasmid

#47069

Purpose

FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), AVI-tag, and…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGM34DEST

Plasmid

#74756

Purpose

Encodes for the 5X repeat of the QUAS response element, followed by the delta pes-10 minimal promoter

Depositor

Keith Yamamoto, Jordan Ward

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

Gateway cassette

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDJSVT87

Plasmid

#109302

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Use

Tags

OmpA 1-27, 6His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pBXCA3GH

Plasmid

#47071

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), Avi-tag, GFP…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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BBa_J72113-BBa_J72103

Plasmid

#40785

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi + cin + repL + pacA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pICXNH3

Plasmid

#49020

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCHAP4

Plasmid

#206850

Purpose

A plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

Plasmid

#164263

Purpose

Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Arabinose

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTOX2

Plasmid

#127447

Purpose

Encodes MqsR toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

mqsR toxin

Use

Tags

Expression

Bacterial

Mutation

Promoter

pRha

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pVHed05

Plasmid

#82351

Purpose

Contains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetR

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

TP901-1 integrase
Bxb1 integrase
AraC (araC )
TetR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPt_Cp

Plasmid

#206855

Purpose

A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pEMF037

Plasmid

#157634

Purpose

Batis maritima MHT

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

MHT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEpic_Lite

Plasmid

#84373

Purpose

3rd-generation lentiviral destination vector for gateway cloning

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72100

Plasmid

#40781

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

lacZ

Use

Synthetic Biology; Phagemid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGL0_142 [EilR-PjexD]

Plasmid

#199026

Purpose

Level 0 part

Depositor

Nili Ostrov, Cultivarium Tools, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

EilR-PjexD

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCD227

Plasmid

#113318

Purpose

AraC-pBAD-dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Tags

Expression

Bacterial

Mutation

R93A

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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JBEI-12050

Plasmid

#84982

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

MevTo-T21-MK-aphA

Use

Tags

Expression

Bacterial

Mutation

Promoter

LacUV5

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGM48DEST

Plasmid

#74757

Purpose

Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

Depositor

Keith Yamamoto, Jordan Ward

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

QS
Gateway cassette

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge