Skip to main content
Addgene

pVHed05
(Plasmid #82351)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82351 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    ColE1
  • Backbone size w/o insert (bp) 2200
  • Total vector size (bp) 7352
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TP901-1 integrase
  • Insert Size (bp)
    1527
  • GenBank ID

Gene/Insert 2

  • Gene/Insert name
    Bxb1 integrase
  • Insert Size (bp)
    1503

Gene/Insert 3

  • Gene/Insert name
    AraC
  • Insert Size (bp)
    878
  • Entrez Gene
    araC (a.k.a. b0064, ECK0065)

Gene/Insert 4

  • Gene/Insert name
    TetR
  • Insert Size (bp)
    624

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is exactly the Dual-recombinase-controller (Addgene 44456) from Bonnet et al, with the addition of a constitutive wild type tetR gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVHed05 was a gift from Richard Murray (Addgene plasmid # 82351 ; http://n2t.net/addgene:82351 ; RRID:Addgene_82351)
  • For your References section:

    A population-based temporal logic gate for timing and recording chemical events. Hsiao V, Hori Y, Rothemund PW, Murray RM. Mol Syst Biol. 2016 May 17;12(5):869. doi: 10.15252/msb.20156663. PubMed 27193783