pVHed05
(Plasmid
#82351)
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PurposeContains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 82351 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneColE1
- Backbone size w/o insert (bp) 2200
- Total vector size (bp) 7352
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameTP901-1 integrase
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Insert Size (bp)1527
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GenBank ID
Gene/Insert 2
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Gene/Insert nameBxb1 integrase
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Insert Size (bp)1503
Gene/Insert 3
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Gene/Insert nameAraC
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Insert Size (bp)878
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Entrez GenearaC (a.k.a. b0064, ECK0065)
Gene/Insert 4
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Gene/Insert nameTetR
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Insert Size (bp)624
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is exactly the Dual-recombinase-controller (Addgene 44456) from Bonnet et al, with the addition of a constitutive wild type tetR gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVHed05 was a gift from Richard Murray (Addgene plasmid # 82351 ; http://n2t.net/addgene:82351 ; RRID:Addgene_82351) -
For your References section:
A population-based temporal logic gate for timing and recording chemical events. Hsiao V, Hori Y, Rothemund PW, Murray RM. Mol Syst Biol. 2016 May 17;12(5):869. doi: 10.15252/msb.20156663. PubMed 27193783