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Plasmid#186686PurposeExpresses SNAP-23 in E. ColiDepositorInsertSNAP23
UseTagsGST and thrombin siteExpressionBacterialMutationPromoterT7Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Stx3
Plasmid#186685PurposeExpresses Syntaxin 3 in E. ColiDepositorInsertSyntaxin 3
UseTags6 his tagExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-psbA2 point mutation
Plasmid#182929Purposepoint mutation on psbA2 by CRISPR in Synechocystis 6803DepositorInsertsddcpf1
gRNA targeting psbA2 in Synechocystis 6803: gatcttcggtcgcttgatctttc
psbA
UseCRISPRTagsExpressionMutationS264A, and silent mutation to remove PAM sitePromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK617
Plasmid#179297PurposeExpresses dCas9 fused to VP64DepositorInsertdCas9-VP64
UseTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterCAGAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL30
Plasmid#174064PurposeEncodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.DepositorInsertpGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL15
Plasmid#174069PurposeExpresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)
Plasmid#173462PurposeC-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004)
Plasmid#173460PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-mNeonGreen (pJJF003)
Plasmid#173459PurposeC-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timerDepositorInsertmCherry-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002)
Plasmid#173458PurposeC-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJBL714
Plasmid#140384PurposeExpresses CtcS-regulated 3WJdB RNA aptamer driven by T7 promoter with the ctcO sequence 5BP downstream from the promoterDepositorInsertT7-ctcO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL720
Plasmid#140390PurposeExpresses QacR-regulated 3WJdB RNA aptamer driven by T7 promoter with the qacO sequence 5BP downstream from the promoterDepositorInsertT7-qacO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL722
Plasmid#140392PurposeExpresses TtgR-regulated 3WJdB RNA aptamer driven by T7 promoter with the ttgO sequence 5BP downstream from the promoterDepositorInsertT7-ttgO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-TMED3-Reverse-Complement
Plasmid#136018PurposeThe Reverse Complement of TMED3 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorInsertTMED3 3'UTR (Reverse Complement) (TMED3 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-ZC3H15-Reverse-Complement
Plasmid#136019PurposeThe Reverse Complement of ZC3H15 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorInsertZC3H15 3'UTR (Reverse Complement) (ZC3H15 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSKB3SaGGR I206F (JBEI-3678)
Plasmid#110149PurposeSaGGR site-directed mutation of I206FDepositorInsertGGR
UseTagsExpressionBacterialMutationSaGGR site-directed mutation of I206FPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3SaGGR G91H (JBEI-3677)
Plasmid#110148PurposeSaGGR site-directed mutation of G91HDepositorInsertGGR
UseTagsExpressionBacterialMutationSaGGR site-directed mutation of G91HPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3SaGGR L377H (JBEI-3683)
Plasmid#110150PurposeSaGGR site-directed mutation of L377HDepositorInsertGGR
UseTagsExpressionBacterialMutationSaGGR site-directed mutation of L377HPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTN177E-myc
Plasmid#107474PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation N177E with approximately wildtype activityDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationR596QPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTY289I-myc
Plasmid#107480PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y289I with approximately wildtype activityDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationN177EPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only