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pJEP220-AAV-0.5Synapsin-GFP-pA

Plasmid

#62920

Purpose

AAV vector backbone designed to express GFP from a 0.5Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

0.5synapsin

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP218-AAV-1.3αCaMKII-GFP-pA

Plasmid

#62922

Purpose

AAV vector backbone designed to express GFP from a 1.3CaMKIIa promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

1.3αCaMKII

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP219-AAV-1.1Synapsin-GFP-pA

Plasmid

#62921

Purpose

AAV vector backbone designed to express GFP from a 1.1Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

1.1Synapsin

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP221-AAV-RSV-GFP-pA

Plasmid

#62919

Purpose

AAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

Rous Sarcoma Virus(RSV)

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV CAG-DO-TC66T-mCherry

Plasmid

#175178

Purpose

Cre-off TVA-mCherry fusion protein with 66T mutation

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

TVA

Use

AAV

Tags

mCherry

Expression

Mutation

E66T

Promoter

CAG

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

Plasmid

#172200

Purpose

AAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

Depositor

Garrett Kaas

Article

Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

Insert

HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

Use

AAV and Mouse Targeting; Tale

Tags

HA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

transcription regulatory domain removed

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-ChromeT-mCherry

Plasmid

#123126

Purpose

AAV-mediated expression of ChromeT-mCherry under the GFAP promoter.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

Promoter

GFAP104

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirMecp2

Plasmid

#163704

Purpose

pAAV plasmid to induce expression of micro-RNA targeted against mouse Mecp2 and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirMecp2 and EGFP (Mecp2 Mouse, Aequorea victoria)

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirNega

Plasmid

#163705

Purpose

pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirNega and EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-YPET-C1-WPRE

Plasmid

#159954

Purpose

untargeted marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

YPET-C1

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mScarlet-C1-WPRE

Plasmid

#159955

Purpose

untargeted marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mScarlet-C1

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E1-dTom-nlsdTom

Plasmid

#135637

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E1 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E3-dTom-nlsdTom

Plasmid

#135638

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E3 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E4-dTom-nlsdTom

Plasmid

#135639

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E4 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E10-dTom-nlsdTom

Plasmid

#135645

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E10 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mIsl2-EGPF

Plasmid

#153191

Purpose

Mouse Isl2 (mIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1 intron 1 gRNA as2

Plasmid

#132394

Purpose

Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r85

Plasmid

#133414

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 85

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r78 (Vmn1r78 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP5g-P2A-Vmn1r89

Plasmid

#133416

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 89

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r89 (Vmn1r89 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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p-AAV2-hSyn-MerMAID1-Citrine

Plasmid

#126520

Purpose

Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

Insert

MerMAID1

Use

AAV

Tags

Citrine

Expression

Mutation

Promoter

human synapsin

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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