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Showing: 2241 - 2260 of 2313 results

  5. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

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  7. LacI-pLacI-pIII pm

    Plasmid
    #218100
    Purpose
    Phagemid that expresses LacI, and pIII under the Lac promoter
    Depositor
    Kevin Esvelt
    Insert
    pIII
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac promoter
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218100-plasmid-map-sequence-id-432442

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  10. pH3mCHSH2

    Plasmid
    #101053
    Purpose
    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.
    Depositor
    Jennifer Lodge
    Article
    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108
    Insert
    Cryptococcus mCherry expression plasmid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Cryptococcus Histone3 promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    101053-plasmid-map-sequence-id-192942

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  11. SELP-bio-His

    Plasmid
    #51639
    Purpose
    Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SELP (SELP Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51639-plasmid-map-sequence-id-78961

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  15. DT034A

    Plasmid
    #159395
    Purpose
    Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
    Use
    Synthetic Biology
    Tags
    FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…
    Expression
    Bacterial
    Mutation
    Promoter
    J23117 and pLux promoter
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    159395-plasmid-map-sequence-id-393061

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  17. DT050A

    Plasmid
    #159411
    Purpose
    Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    J23101 promoter
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    159411-plasmid-map-sequence-id-326574

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  18. pTNos:BASTA:PNos (GB0023)

    Plasmid
    #68211
    Purpose
    Provides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    bar gene
    Use
    Unspecified
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    PNos
    Available since
    Oct. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68211-plasmid-map-sequence-id-119063

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Showing: 2241 - 2260 of 2313 results