DT050A
(Plasmid
#159411)
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PurposeConstitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB1A3
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Backbone manufactureriGEM parts registry
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepr.J23101-BDNF (Bdnf, Mouse)
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SpeciesM. musculus (mouse), Synthetic
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Entrez GeneBdnf
- Promoter J23101 promoter
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer VF2 primer (iGEM parts registry)
- 3′ sequencing primer VR primer (iGEM parts registry) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DT050A was a gift from Sheref Mansy (Addgene plasmid # 159411 ; http://n2t.net/addgene:159411 ; RRID:Addgene_159411) -
For your References section:
Artificial cells drive neural differentiation. Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet ML, Mansy SS. Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 10.1126/sciadv.abb4920. Print 2020 Sep. 10.1126/sciadv.abb4920 PubMed 32948587