promoter J23110 (upstream of RBS, mRFP1)
(Plasmid
#117852)
-
PurposeFor course in "Synthetic Biology: A Lab Manual"
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 117852 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneBioBrick J61002 vector
-
Backbone manufactureriGEM Registry
-
Modifications to backboneBioBrick sites removed
-
Vector typeSynthetic Biology ; Escherichia coli
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemedium-high promoter, RBS, mRFP1
-
SpeciesSynthetic
-
MutationBioBrick sites removed
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
promoter J23110 (upstream of RBS, mRFP1) was a gift from Anthony Forster (Addgene plasmid # 117852 ; http://n2t.net/addgene:117852 ; RRID:Addgene_117852) -
For your References section:
Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. J Biol Eng. 2018 May 10;12:8. doi: 10.1186/s13036-018-0100-0. eCollection 2018. 100 [pii] PubMed 29760772