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Plasmid#204686PurposeCadmium responsive genetic circuitDepositorInsertBxb1 (Bxb1p45 Synthetic)
UseSynthetic BiologyTagsCadRExpressionBacterialMutationPromoterInducible, HspR is its repressorAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Magenta
Plasmid#160446PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Magenta
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTOX3
Plasmid#127448PurposeEncodes Tse2 toxin. CAM-RDepositorInserttse2 toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB152
Plasmid#101663PurposeOpto-T7RNAP*(563-F2)DepositorInsertOpto-T7RNAP*(563-F2)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSM040
Plasmid#128294Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Violet
Plasmid#160447PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Violet
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB203
Plasmid#101675PurposeOpto-T7RNAP(563-F1)DepositorInsertOpto-T7RNAP(563-F1)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GFPM2
Plasmid#102791PurposeExpresses GFP under WT T7 promoterDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCasX1
Plasmid#87685Purposep15A plasmid expressing CasX locus from DeltaproteobacteriaDepositorInsertCasX1
UseTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBsaBM3
Plasmid#85102PurposeP450 BM3 variant (A74G/C773S/C810S)DepositorInsertP450 BM3
UseCloning vectorTagsExpressionMutationA74G/C773S/C810SPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-HA-LRR-NEL-C (B3-B4)
Plasmid#211860PurposeCDS for E3-DART ligase sequence in domestication vectorDepositorInsertLRR-NEL
UseSynthetic BiologyTagsHAExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-HA-LRR-NELC492A-C (B3-B4)
Plasmid#211862PurposeMutant E3-DART ligase in domestication vectorDepositorInsertLRR-NEL
UseSynthetic BiologyTagsHAExpressionMutationC492APromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Yellow
Plasmid#160443PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Yellow
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-mCherry-AsLOV2*(543)
Plasmid#202712PurposeExpresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)DepositorInsertmCherry-AsLOV2*(543)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTOX1
Plasmid#127446PurposeEncodes YhaV toxin. CAM-RDepositorInsertyhaV toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM489-Snap
Plasmid#108437PurposeORBIT integrating plasmid for C-terminal tagging with SNAP tag.DepositorInsertattB-SNAP tag
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBXCA3H
Plasmid#47072PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), Avi-tag, and…ExpressionBacterialMutationPromoteraraBADAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPTK021-7-PARS
Plasmid#84990PurposeType 7, Pichia autonomously replicating sequenceDepositorInsertPichia autonomously replicating sequence
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRN110
Plasmid#84462PurposepJB38-NWMN29-30 + SarA_P1-mKate2-TermDepositorInsertSarA_P1-mKate2 from pRN11
UseTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG86-J23151-LasR
Plasmid#102456PurposeExpresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.DepositorInsertLasR (lasR )
UseTagsExpressionBacterialMutationPromoterJ23151Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only