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MUT_S1-spacer-H1
Plasmid#218399Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1,NR
Plasmid#218395Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_VP12A
Plasmid#212988PurposeEncodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus type I Mahoney VP12A
UseMammalian cell free protein expressionTagsHA tagExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Venus-30aa-Noxa-4E-pEGFP-C1
Plasmid#167529PurposeTo increase the length of the linker between Venus and Noxa-4E proteinsDepositorInsertPMA-induced protein 1 (PMAIP1 Human)
UseTagsVenusExpressionMammalianMutationC25E, L29E, F32E, L36EPromoterCMVAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK573
Plasmid#72456PurposeProduces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationsynthetic gene with ~57 silent substitutionsPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK569
Plasmid#72455PurposeProduces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationsynthetic gene with ~51 silent substitutionsPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPS1494
Plasmid#1377DepositorInsertpGAL-REV-GFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS314-T7-GFP
Plasmid#33130DepositorInsertGFP with TDH3 5' and 3' UTR (gfp )
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFR_HCV_xb
Plasmid#11510DepositorInsertFirefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET11d-RAN
Plasmid#108919PurposeExpresses human RANDepositorInsertRAN (RAN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET/ELP-I-CAT
Plasmid#71461PurposeELP-intein purification systemDepositorTypeEmpty backboneUseTagsELP ((VPGXG)110) and inteinExpressionBacterialMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT416
Plasmid#8607DepositorInsertbarnase, barstar
UseTagsphoA signal sequence to barnaseExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2 SENP2
Plasmid#13382DepositorInsertSENP2 (SENP2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro Scr shRNA
Plasmid#30520DepositorInsertScr shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pReadthroughUGA
Plasmid#11513DepositorInsertFirefly luciferase, UAA stop codon, Renilla luciferase, and 3' UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pReadthroughUAA
Plasmid#11512DepositorInsertFirefly luciferase, UAA stop codon, Renilla luciferase, and 3' UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pReadthroughUAG
Plasmid#11511DepositorInsertFirefly luciferase, UAG stop codon, Renilla luciferase, and 3' UTR short RNA binding sites
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro GFP shRNA
Plasmid#30519DepositorInsertGFP shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT7EGFPgRNA
Plasmid#46760DepositorInsertegfp target
UseCRISPRTagsExpressionMutationPromoterT7Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only
