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Plasmid#112100PurposeC:G-to-T:A base editingDepositorInsertAncBE4max_P2A_GFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
DD-Cas9 with filler sequence and Venus (EDCPV)
Plasmid#90085PurposeThis plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNADepositorInsertCas9
UseCRISPR and LentiviralTagsDestabilized Domain and FlagExpressionMammalianMutationPromoterEFSAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
UseTagsHalo and His6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
35S-eGFP-nosT
Plasmid#80127PurposeTransient expression vector for eGFP in plantsDepositorTypeEmpty backboneUseTagseGFPExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMD2-VSVGmut
Plasmid#182229PurposeExpresses VSVGmut protein in mammalian cells for lentiviral pseudotypingDepositorInsertVSVGmut
UseTagsExpressionMammalianMutationK47Q, R354APromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-SopF
Plasmid#135174PurposeExpresses an mCherry-SopF fusion in mammalian cellsDepositorInsertSopF (STM1239 Salmonella enterica serovar Typhimurium)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Venus-Akaluc (neo)
Plasmid#124701PurposeAka-Luciferase reporterDepositorInsertVenus-Akaluciferase
UseLentiviralTagsExpressionMutationPromoterhPGKAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP
Plasmid#177825PurposeHost Cell Reactivation (HCR) control GFP plasmid (pMax backbone)DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pmGFP-ADAR1-p150
Plasmid#117927PurposeADAR1-p150 expression plasmid.DepositorInsertADAR1-p150 (ADAR Human)
UseTagsmyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDY0663 ACTB atgRNA with v2 scaffold
Plasmid#179108PurposeACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffoldDepositorInsertACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW63.YY1.FKBP.knock-in.BFP
Plasmid#104371PurposeFor knocking in FKBP F36V P2A BFPDepositorInsertFKBP12(F36V) -P2A-BFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2
Plasmid#14129DepositorInsertTSC2 (TSC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 YFP
Plasmid#84911Purposeexpression of TDP-43 YFP in mammalian cellsDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA1er
Plasmid#58216PurposeRed fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertR-CEPIA1er
UseTagsmycExpressionMammalianMutationR-GECO1 E300D M305L D329N F332I E336D N346K F361W…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau
Plasmid#46904Purposeexpresses EGFP tagged Tau in mammalian cellsDepositorInsertMAPT (MAPT Human)
UseTagsEGFPExpressionMammalianMutationThis WT htau construct contains human four-repeat…PromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTP396
Plasmid#149336PurposeBacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobodyDepositorInsertanti-GFP nanobody
UseTagsBiotin acceptor peptide (Avi-tag), His14, and SUM…ExpressionBacterialMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only