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Plasmid#104543PurposePiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoterDepositorInsertTet-On 3G
UseTagsExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112924PurposepiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-EGFP
Plasmid#221553PurposeExpresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
PBCAG-EGFR
Plasmid#40974DepositorInsertEGFR (EGFR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
1137=Hsp70Bb-Cas9[dsRed]
Plasmid#149424PurposeThe piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)DepositorInsertCas9
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD
Plasmid#162804PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI_IRES_Blasticidin
UsePiggybacTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Tfe3-pgk-hph
Plasmid#48755PurposePiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selectionDepositorInsertTfe3 (Tcfe3 Mouse)
UsePiggybacTagsExpressionMutationPromoterCAGAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1
Plasmid#79912PurposepiggyBac transposon vector, Cerulean-2A-sHRPbNLG1DepositorInsertCerulean-2A-sHRPbNLG1
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta
Plasmid#79913PurposepiggyBac transposon vector, Zeocin-2A-sHRPaNRX3betaDepositorInsertZeocin-t2A-sHRPbNRX3beta
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
S7_SwaI_Zeo_PacI
Plasmid#128471PurposeZeo Resistance geneDepositorInsertZeocin Resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_Gag-EIF4E-TM
Plasmid#212658PurposepiggyBac vector to express an MLV Gag-EIF4E fusion protein (W56L, W102L, W166L mutations installed into EIF4E)DepositorArticleInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UsePiggybacTagsEIF4E (W56L, W102L, W166L)ExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD
Plasmid#162805PurposeSTELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI-HA_IRES_Blasticidin
UsePiggybacTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLR5-U6-sgRNA-EF
Plasmid#82635PurposepiggyBac vector for expressing modified sgRNA in mammalian cellsDepositorTypeEmpty backboneUseCRISPR; Piggybac vectorTagsExpressionMammalianMutationPromoterU6Available sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
S9_attL1-EGFP-C-term-attL2
Plasmid#128473PurposeEGFP entry cloneDepositorInsertEGFP entry clone
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro
Plasmid#168806PurposePiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expressionDepositorInsertASCL1-P2A-DLX2-T2A-mCherry
UseSynthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Tfe3::ERT2-pgk-hph
Plasmid#48756PurposePiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selectionDepositorInsertTfe3::ERT2 (Tcfe3 Mouse)
UsePiggybacTagsERT2ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
KX002: pMVP/PB-DEST
Plasmid#121869PurposepMVP destination vector, empty PiggyBac transposon vector backboneDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-Sox17
Plasmid#60669PurposePiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoterDepositorInsertSox17 (Sox17 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-Tal1
Plasmid#60670PurposePiggyBac expression vector encoding murine Tal1 downstream of a doxycycline inducible promoterDepositorInsertTal1 (Tal1 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-Mycn
Plasmid#60667PurposePiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoterDepositorInsertMycn (Mycn Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only