Purpose
Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.
Inserts
Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
Flagellin
Use
Synthetic Biology
Tags
Expression
Bacterial
Mutation
Changed Threonine 248 to TAG stop-codon.
Promoter
Pseudomonas aeruginosa Leu-tRNA native promoter a…
Available since
June 14, 2021
Availability
Academic Institutions and Nonprofits only