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Showing: 201 - 220 of 18276 results

  1. pBGE1.0

    Plasmid
    #165986
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    165986-plasmid-map-sequence-id-375342

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  2. pDGB3omega1_KanR_BastaR

    Plasmid
    #186426
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186426-plasmid-map-sequence-id-369066

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  3. pAGE1.0

    Plasmid
    #165983
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Spec resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    165983-plasmid-map-sequence-id-378731

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  5. A1-LCD Aro+

    Plasmid
    #169717
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, aromatic amino acids. PHE and TYR amino acids shuffled to optimize mixing.
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169717-plasmid-map-sequence-id-334791

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  6. pJK210

    Plasmid
    #73325
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73325-plasmid-map-sequence-id-135033

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  7. pJK265

    Plasmid
    #73598
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioC
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73598-plasmid-map-sequence-id-136140

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  8. pJK261

    Plasmid
    #73323
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioA with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioA
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73323-plasmid-map-sequence-id-135031

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  9. pJK196

    Plasmid
    #73322
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioD
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73322-plasmid-map-sequence-id-135030

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  10. pJK266

    Plasmid
    #73326
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioD
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73326-plasmid-map-sequence-id-135034

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  11. pJK195

    Plasmid
    #73321
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioC
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains silent mutation in Val428
    Promoter
    T7
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73321-plasmid-map-sequence-id-135029

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  12. pJK234

    Plasmid
    #73324
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC
    Depositor
    T. J. Kappock
    Inserts
    violacein biosynthesis protein VioA
    violacein biosynthesis protein VioB
    violacein biosynthesis protein VioC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73324-plasmid-map-sequence-id-135032

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  13. pJK021

    Plasmid
    #75060
    Purpose
    Produces Saccharomyces cerevisiae ADE2 (N5-carboxyaminoimidazole ribonucleotide mutase and N5-carboxyaminoimidazole ribonucleotide synthetase fusion protein)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase ADE2 (ADE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E. coli optimized codons in first 25 residues
    Promoter
    lac
    Available since
    Availability
    Academic Institutions and Nonprofits only
    75060-plasmid-map-sequence-id-142245

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  18. CoV2-Spike-D614G-Beta

    Plasmid
    #177962
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)
    Depositor
    Melanie Ott
    Article
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Insert
    SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y, D614G
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177962-plasmid-map-sequence-id-348261

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  19. CoV2-Spike-D614G-Epsilon

    Plasmid
    #177964
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G)
    Depositor
    Melanie Ott
    Article
    Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.
    Insert
    SARS-CoV-2 Spike protein (Epsilon: S13I, W152C, L452R, D614G) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S13I, W152C, L452R, D614G
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177964-plasmid-map-sequence-id-348252

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Showing: 201 - 220 of 18276 results