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CROPseq-Guide-Puro-mCherry2

Plasmid

#219679

Purpose

CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

Depositor

Christoph Bock

Article

Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-MAPPER

Plasmid

#117721

Purpose

MAPPER Selectively Labels ER-PM Junctions

Depositor

Jen Liou

Article

Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

Insert

MAPPER

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1

Plasmid

#20066

Purpose

Bacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Denisov et al J Biol Chem. 2007 Mar 9. 282(10):7066-76

Insert

MSP1E3D1

Use

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeats of …

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL6-t2A-BCL2

Plasmid

#135305

Purpose

Overexpression of multiple human genes (BCL2 and BCL6)

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

BCL6 (BCL6 Human)
BCL2 (BCL2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-JAK2

Plasmid

#23915

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

JAK2 (JAK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mCherry

Plasmid

#155222

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pShuttle-mRFP-gsk3 s9a

Plasmid

#24371

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

GSK3beta(S9A) (GSK3B Human)

Use

Adenoviral

Tags

mRFP

Expression

Mammalian

Mutation

S9A. Mutant version of GSK3 that lacks the inhibi…

Promoter

Available since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

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mCherry2-N1

Plasmid

#54517

Purpose

Localization: N1 Cloning Vector, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pPTP1BD181A-mCherry

Plasmid

#40270

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

PTPN1 (PTPN1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D181A

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pTW1169

Plasmid

#236583

Purpose

TRV1

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

TRV1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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murine TCR OTI-2A.pMIG II

Plasmid

#52111

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

T2A (from Thosea asigna virus)

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP670nano3

Plasmid

#184664

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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FUGW-PercevalHR

Plasmid

#49083

Purpose

Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCVD442

Plasmid

#11074

Depositor

Michael Donnenberg, James Kaper

Article

Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

Insert

sacB (sacB Bacillus subtilis)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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pRGEB31

Plasmid

#51295

Purpose

Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into plants by agrobacterium mediated transformation.

Depositor

Yinong Yang

Article

Xie et al Mol Plant. 2013 Nov;6(6):1975-83. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Rice snoRNA U3 and dual 35S promoter

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA Donor_hEF1a

Plasmid

#229779

Purpose

Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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ABE8.20-m

Plasmid

#136300

Purpose

expresses ABE8.20-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-m

Use

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red

Plasmid

#48249

Purpose

Genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G3BP1-DeltaRBP

Plasmid

#135999

Purpose

G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C-term of G3BP1 Deleted for Delta RBP

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/EV

Plasmid

#120848

Purpose

Control vector, 20nt random sequence between gateway recombination sites

Depositor

Jan Rehwinkel

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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