-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 24371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepShuttle-CMV
-
Backbone manufacturerAgilent Technologies
- Backbone size w/o insert (bp) 7469
-
Vector typeMammalian Expression, Adenoviral
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameGSK3beta(S9A)
-
Alt nameGSK3beta
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2000
-
MutationS9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
-
Entrez GeneGSK3B
-
Tag
/ Fusion Protein
- mRFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV-F, EGFP-C (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bycDNA obtained from Niels Galjart
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pShuttle-mRFP-gsk3 s9a was a gift from Torsten Wittmann (Addgene plasmid # 24371 ; http://n2t.net/addgene:24371 ; RRID:Addgene_24371) -
For your References section:
GSK3beta phosphorylation modulates CLASP-microtubule association and lamella microtubule attachment. Kumar P, Lyle KS, Gierke S, Matov A, Danuser G, Wittmann T. J Cell Biol. 2009 Mar 23. 184(6):895-908. 10.1083/jcb.200901042 PubMed 19289791