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pAAV-U6-sgRNA-CMV-GFP

Plasmid

#85451

Purpose

Express sgRNA in mammalian cells

Depositor

Hetian Lei

Article

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Insert

pCMV-EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

pCMV-EGFP

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBOB-EF1-FastFUCCI-Puro

Plasmid

#86849

Purpose

3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

Depositor

Duncan Jodrell, Kevin Brindle

Article

Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

Inserts

mKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1 and PGK

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-Sec61β

Plasmid

#172442

Purpose

Expression of ER protein Sec61beta with an N-terminal FKBP-GFP tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Protein transport protein Sec61 subunit beta

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUCBB-eGFP

Plasmid

#32548

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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eeBxb1

Plasmid

#222339

Purpose

Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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HPV16 E7 TCR

Plasmid

#122728

Purpose

Express HPV16 E7 TCR

Depositor

Christian Hinrichs

Article

Jin et al JCI Insight. 2018 Apr 19;3(8). pii: 9948

Insert

HPV16 E7 TCR

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3Luc-5XNF-kappaB

Plasmid

#185695

Purpose

Reporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').

Depositor

Esther López-Bayghen

Article

Hernandez-Melchor et al ASN Neuro. 2022 Jan-Dec;14:1759091422111

Insert

NF-kappaB (NFKB1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

NF-kappaB binding site

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-GFP

Plasmid

#11680

Depositor

Geoff Wahl

Article

Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

Insert

Human histone H2B (H2BC11 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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pLX311 Luciferase

Plasmid

#117735

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCre

Plasmid

#236212

Purpose

Arabinose-inducible Cre recombinase

Depositor

Matthew Waldor

Article

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

Cre recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-SpCas9

Plasmid

#104588

Purpose

AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Insert

Streptococcus pyogenes Cas9

Use

AAV and CRISPR

Tags

Myc

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLZts

Plasmid

#128799

Purpose

Temperature-sensitive vector for allelic replacement mutagenesis of Group A Streptococcus

Depositor

Timothy Barnett

Article

Barnett et al Methods Mol Biol. 2020;2136:59-69. doi:

Type

Empty backbone

Use

Bacterial mutagenesis

Tags

Expression

Mutation

Promoter

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CXCR4

Plasmid

#98942

Purpose

Mammalian expression plasmid for untagged human CXCR4

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

Tags

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HRE-dUnaG

Plasmid

#124372

Purpose

UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

Depositor

Roland Friedel

Article

Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

Insert

HRE-dUnaG

Use

Lentiviral

Tags

PEST-degron and Myc tag

Expression

Mutation

Promoter

HRE (HIF responsive element 5x + CMV minimal prom…

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-mt-mKeima

Plasmid

#131626

Purpose

lentiviral expression of mitochondrial mKeima for generation of stable cell lines

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Lifeact-EGFP

Plasmid

#58470

Purpose

Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Lifeact

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9

Plasmid

#42230

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Has Service

Cloning Grade DNA

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

Plasmid

#221899

Purpose

Expresses alpha tubulin fused to mStayGold

Depositor

Janelia Integrative Imaging

Insert

mStayGold-aTubulin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-3XHA-EGFP-OMP25

Plasmid

#83356

Purpose

For tagging mitochondria with HA epitopes (HA-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XHA-EGFP-OMP25

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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