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COC053

Plasmid

#209131

Purpose

Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

Depositor

Christopher A. O'Callaghan

Article

O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

Insert

Enzyme BirA (birA E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pHBS1084 GFP-TDP43 deltaCTD

Plasmid

#118798

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

Deletion of CTD in full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with LFn + Fla

Plasmid

#84866

Purpose

Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Use

Baculovirus

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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TS-PE2-N

Plasmid

#176649

Purpose

Expresses N-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

N-terminus of PE2
Artificial SD

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV and None

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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DDX5 D248N V5

Plasmid

#199577

Purpose

Expresses helicase dead DDX5 cloned into pLenti puro

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

DDX5 (DDX5 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

D248N

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1-N100 S449A/T450A/S454A

Plasmid

#19849

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1-N100 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

deleted amino acids 1-100 and changed Serine 449,…

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40571

Depositor

Daniel Gerlich

Article

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor N-terminally fused to histone 2B

Use

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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UBQLN2_450-624W

Plasmid

#176852

Purpose

Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells

Depositor

Carlos Castañeda

Article

Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6.

Insert

UBQLN2_450C_W (UBQLN2 Human)

Use

Tags

Expression

Bacterial

Mutation

deletion of residues 1-449 and addition of C-term…

Promoter

T7

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHBS1413 GFP-TDP43 VLIM-F

Plasmid

#118802

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

All VLIM to F in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1411 GFP-TDP43 FYW-S

Plasmid

#118800

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

All FYW to S in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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DsRed +9e

Plasmid

#191766

Purpose

Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Use

Tags

N terminal fusion of positively charged polypetod…

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5-ERRbeta-delta10

Plasmid

#52187

Purpose

Expresses human ERRbeta-delta10 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Use

Tags

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
pHBS1415 GFP-TDP43 VLIM-S

Plasmid

#133323

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

ALL VLIM in the TDP43 IDRs mutated to S

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(K271Q)

Plasmid

#133270

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )

Use

Oocyte expression

Tags

Expression

Mutation

K271Q

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#133077

Purpose

X. laevis expression vector. It will generate the human TRAAK channel

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)

Use

Tags

Expression

Mutation

Q258K

Promoter

T7

Available since

Oct. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj11

Plasmid

#173139

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj11 (kcnj11 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with LFn + PrgJ

Plasmid

#84869

Purpose

Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
PrgJ

Use

Baculovirus

Tags

6xHis

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pHBS1412 GFP-TDP43 FYW-L

Plasmid

#118801

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

All FYW to L in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAT9750-BEAR-mCherry

Plasmid

#162990

Purpose

BEAR target plasmid with split mCherry and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcna1a-IRES-EGFP

Plasmid

#164965

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcna1a (kcna1a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Expression

Mutation

Promoter

actinb (actb2) promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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