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Plasmid#102419PurposeExpresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.DepositorInsertLGALS3 (LGALS3 Human)
UseTagsmAzami-greenExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5
Plasmid#97044PurposeExpresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertLigand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
UseLentiviralTagsExpressionMammalianMutationPromoterTet onAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSc1-DD
Plasmid#80439PurposeExpresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsE. coli dihydrofolate reductaseExpressionMammalianMutationPromoterCMV and U6Available sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)
Plasmid#208951PurposeA variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.DepositorInsertTGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)
Plasmid#208950PurposeA variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.DepositorInsertFBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)
Plasmid#208949PurposeA variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.DepositorInsertRepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-TraI-nCas9-EcKlenow (JO604)
Plasmid#208948PurposeA variant CE1 construct with TraI HUH endonuclease, expressed from CMV or T7 promoters.DepositorInsertTraI-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
PTRF/Cavin1_L (OZ513)
Plasmid#27188DepositorInsertZinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-E112A-FL
Plasmid#110218PurposeExpression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsHis6 and ZBExpressionBacterialMutationE112A, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSc1
Plasmid#80436PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6Available sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSc2
Plasmid#80437PurposeExpresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
NET23 pEGFP-N2 (1174)
Plasmid#62037Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET23 (STING1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NFATc1_L (OZ543)
Plasmid#28068DepositorInsertZinc finger array targeting NFATc1 (nfatc1 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
NFATc1_R (OZ544)
Plasmid#28069DepositorInsertZinc finger array targeting NFATc1 (nfatc1 Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTN-Y117C-FL
Plasmid#110219PurposeExpression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsHis6 and ZBExpressionBacterialMutationY117C, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTRF/Cavin1_R (OZ514)
Plasmid#27189DepositorInsertZinc finger array targeting PTRF/Cavin1 (cavin1b Zebrafish)
UseZebrafish targetingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pStreptagII 3C EGFR EABR
Plasmid#234994PurposeFor production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminusDepositorInsertEGFR-EABR (EGFR Human)
UseTagsStreptag-II, HRV 3C siteExpressionMammalianMutationPromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHA StreptagII 3C NIS EABR
Plasmid#234988PurposeFor production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surfaceDepositorInsertNIS-EABR (SLC5A5 Human)
UseTagsHA, Streptag-II, HRV 3C siteExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-StCV-nCas9-EcKlenow (JO1269)
Plasmid#217790PurposeVariant CE1 construct with StCV HUH endonuclease, expressed from CMV or T7 promoters.DepositorInsertStCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
HUHe variant click editor (CE1) - pCMV-T7-CaCV-nCas9-EcKlenow (JO1261)
Plasmid#217791PurposeVariant CE1 construct with CaCV HUH endonuclease, expressed from CMV or T7 promoters.DepositorInsertCaCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only