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Plasmid#17554DepositorInsertFLAG-FoxO1-ADA
UseTagsFLAGExpressionMammalianMutationsiRNA-resistant Foxo1 by replacing 3 residues (un…PromoterAvailable sinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*V106Q)
Plasmid#125651PurposepCMV-ABEmax(TadA E59A, TadA*V106Q) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* V106Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*R47Q)
Plasmid#125657PurposepCMV-ABEmax(TadA E59A, TadA*R47Q) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* R47Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*D108Q)
Plasmid#125655PurposepCMV-ABEmax(TadA E59A, TadA*D108Q) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* D108Q)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta domain 7
Plasmid#40940DepositorInsertEPLIN alpha delta Domain 7 (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 562-600PromoterAvailable sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta domain 3
Plasmid#40937DepositorInsertEPLIN alpha delta Domain 3 (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 106-148PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN beta delta domain 3
Plasmid#40939DepositorInsertEPLIN beta delta domain 3 (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 266-309PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta N54
Plasmid#40934DepositorInsertEPLIN (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 1-54PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta N112
Plasmid#40935DepositorInsertEPLIN (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 1-112PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta N173
Plasmid#40936DepositorInsertEPLIN (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 1-173PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)
Plasmid#221776PurposeExpresses PDGFR-Cry2olig-FRB-P2A-GFP(1-10) component in mammalian cellsDepositorInsertpCMV:: PDGFR-Cry2olig-FRB-P2A-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-ePhi29(+exo) (LM2995)
Plasmid#208966PurposeUnfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.DepositorInsertePhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…PromoterCMV and T7Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)
Plasmid#208974PurposeRecruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)
Plasmid#208973PurposeRecruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)
Plasmid#208971PurposeRecruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-Phi29(+exo) (LM2759)
Plasmid#208964PurposeUnfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-Δ115-133, 204-217 PPM1H
Plasmid#207343PurposeMammalian expression of HA-Δ115-133, 204-217 PPM1HDepositorInsertPPM1H (PPM1H Human)
UseTagsHAExpressionMammalianMutationΔ115-133, Δ204-217PromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag-LRRK2 K17A/K18A/R1441G
Plasmid#186013PurposeFlag-LRRK2 K17A/K18A/R1441GDepositorInsertFlag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
UseTagsExpressionMammalianMutationSee depositor's comments below.PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-3xFLAG-prs-hMIGA2 (170-593)
Plasmid#192833PurposeExpress codon-optimized human MIGA2 fragment 170-593 in Expi293 cellsDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
UseTags3xFLAG-PreScission cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV
Plasmid#169589PurposeTier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTetO7-PCMVmin-2Available sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only