FLAG-ADA add back mutant pCMV5
(Plasmid #17554)

• Depositing Lab: Domenico Accili
• Publication: Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 1700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FLAG-FoxO1-ADA
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Mutation: siRNA-resistant Foxo1 by replacing 3 residues (underlined) in the sequence 5′-ACGGCGGTCTGAACCAGTATA-3′ The Foxo1 siRNA sequence is: 5′-ACGGAGGATTGAACCAGTATA-3′
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ? (unknown if destroyed)
• 3′ cloning site: ? (unknown if destroyed)
• 5′ sequencing primer: ?

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry