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Plasmid#20909PurposepiggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)DepositorInsertGateway Destination Vector
UsepiggybacTagsIRES-bGeoExpressionMammalianMutationPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21d-myc-BirA
Plasmid#109424PurposeBacterial expression of biotin ligase (BirA) with an N-terminal myc epitopeDepositorInsertBirA (birA )
UseTagsmycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN1
Plasmid#180282PurposeCas9 cassette (TU1)DepositorInsertConLS - pPGK1 - Cas9 - tPGK1 - ConR1
UseTagsExpressionBacterialMutationPromoterpPGK1Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NGN2-EMX1 BSD-mApple
Plasmid#182311PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mAppleUseTagsT2A-mycNLS-mAppleExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
gfasPurple chromoprotein
Plasmid#117849PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coliDepositorInsertpromoter, RBS, gfasPurple
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG106 - KA
Plasmid#178041PurposeBacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markersDepositorTypeEmpty backboneUseShuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pB-TopFlash-NanoLucP
Plasmid#204347PurposeTopFlash reporterDepositorInsertTopFlash-NanoLucP
UseLuciferaseTagsNanoLucPExpressionMutationPromoterminiPAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRIGF-IKZF1-V2
Plasmid#69045Purposeexpression of IKZF1 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.DepositorInsertIKZF1 (IKZF1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationsplice variant 2PromoterIRESAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
meffBlue chromoprotein
Plasmid#117845PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coliDepositorInsertpromoter, RBS, meffBlue
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-teLuc c-myc
Plasmid#100026PurposeEnhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)DepositorInsertteLuc, a NanoLuc mutant
UseTagsc-mycExpressionMammalianMutationD19S, D85N, C164HPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cry2FL-VP64
Plasmid#60554PurposeExpresses Cry2FL-VP64 in mammalian cellsDepositorInsertCry2FL-VP64
UseTagsFLAG, HA tag, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG021 (AVAST type 5)
Plasmid#157899PurposeExpresses the AVAST type 5 defense systemDepositorInsertAVAST type 5 (STAND ATPase with N-terminal SIR2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Kv7.1/pcDNA3.1
Plasmid#111452PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)
UseTags8xHis and EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-spe
Plasmid#117236Purposencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickaseDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APPP1-mGFP
Plasmid#196705PurposeAs mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activityDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmCherry and mEGFPExpressionMammalianMutationLys to Val substitution at position 612PromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
gag-MCP-pol
Plasmid#211370PurposePackaging plasmid for v3 PE-eVLP productionDepositorInsertMMLVgag-MCP-pol
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
scOrange chromoprotein
Plasmid#117840PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coliDepositorInsertpromoter, RBS, scOrange
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
JD633
Plasmid#160393PurposeCRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloningDepositorInsertsZmUbi::wheat GRF4-GIF1 chimera
ZmUbi:TaCas9 + TaU6:gRNA
UseBinary vectorTagsExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiCas9-sgVRK1 #1-Blast
Plasmid#199646PurposeExpresses Cas9 and sgRNA guide targeting VRK1DepositorInsertN/A (VRK1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only