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Plasmid#139993PurposeCMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationxCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag YAP127/128/131/381A
Plasmid#27378DepositorInsertYAP (YAP1 Human)
UseTags2xFLAGExpressionMammalianMutationS127/128/131/381APromoterAvailable sinceMarch 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-pegRNA-tetra-com vector
Plasmid#136271PurposeExpresses PE2 and Tetra-com modified pegRNA cassetteDepositorInsertPE2 and pegRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta LIM
Plasmid#40933DepositorInsertEPLIN alpha (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationLIM domain deleted- amino acids 221-295PromoterAvailable sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-EPLIN alpha delta C-term
Plasmid#40930DepositorInsertEPLIN alpha (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 296-600PromoterAvailable sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)
Plasmid#79619PurposeExpresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTags3xFlag-PmCDA1ExpressionMammalianMutationD10A for nickase Cas9PromoterpCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)
Plasmid#79621PurposeExpresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cellsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionMammalianMutationD10A and H840A for nuclease deficient Cas9PromoterpCMVAvailable sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDF0386 pcmv-hudiscas7-11-s1006-gggs-d1221
Plasmid#186983Purposemammalian expression of cas7-11-s1006-gggs-d1221DepositorInsertcas7-11-s1006-gggs-d122
UseTagsExpressionMammalianMutations1006-gggs-d1221 deletionPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0385 pcmv-hudiscas7-11-r1045-gggs-r1122
Plasmid#186982Purposemammalian expression of cas7-11-r1045-gggs-r1122DepositorInsertcas7-11-r1045-gggs-r1122
UseTagsExpressionMammalianMutationr1045-gggs-r1122 deletionPromoterAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-pegRNA-ST2-com-vector
Plasmid#136272PurposeExpresses PE2 and ST2-com modified pegRNA cassetteDepositorInsertPE2, pegRNA scaffold
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAR-R617M,K618M,K632M,K633M
Plasmid#115778PurposeExpresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cellsDepositorInsertAndrogen Receptor (AR Human)
UseTagsExpressionMammalianMutationR617M, K618M ,K632M, K633M, insertion of Q81 and …PromoterCMVAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MYC-Smurf2 (FF29/30A) C716A
Plasmid#24605DepositorInsertSmurf2 (SMURF2 Human)
UseTagsMYCExpressionMammalianMutationF29A, F30A, C716APromoterAvailable sinceMay 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
p169 pCMV/betaAc-EGFP/beta-gal
Plasmid#8388DepositorInsertfloxed EGFP beta-gal
UseCre/Lox; Beta-galTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax(TadA E59A, TadA*E59A)
Plasmid#125649PurposepCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cellsDepositorInsertABEmax(TadA E59A TadA* E59A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-Tagged Clavesin2 (pCMV-Tag2B-Sec14b)
Plasmid#47431DepositorInsertClavesin2 (CLVS2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)
Plasmid#47430DepositorInsertClavesin1 (CLVS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-XPLN aa1-125 (N125)
Plasmid#73378PurposeExpression of the N-terminal region of XPLN (amino acids 1–125, designated N125)DepositorInsertarhgef3 (ARHGEF3 Human)
UseTagsMyc (QKLISEEDL)ExpressionMammalianMutationExpresses N terminal amino acid 1-125 of XPLN (ar…PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock L57E/L113E/W284A
Plasmid#47343Purposetriple mutation used for co-IPDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationL57E/L113E/W284APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MYC-Smurf2 (FF29/30A, Y459A) WT
Plasmid#24606DepositorInsertSmurf2 (SMURF2 Human)
UseTagsMYCExpressionMammalianMutationF29A, F30A, Y459APromoterAvailable sinceMay 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV5-Flag-EPLIN beta delta C term
Plasmid#40931DepositorInsertEPLIN beta (LIMA1 Human)
UseTagsFlagExpressionMammalianMutationC terminus deletedPromoterAvailable sinceDec. 12, 2012AvailabilityAcademic Institutions and Nonprofits only