pCMV-hAR-R617M,K618M,K632M,K633M
(Plasmid
#115778)
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PurposeExpresses human androgen receptor (R617M, K618M, K632M, K633M) in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 115778 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
- Backbone size w/o insert (bp) 4200
- Total vector size (bp) 7300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAndrogen Receptor
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Alt nameAR
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SpeciesH. sapiens (human)
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MutationR617M, K618M ,K632M, K633M, insertion of Q81 and Q82
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert encodes 25 glutamines in the polymorphic region of the androgen receptor. Mutations are based on the numbering from the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-hAR-R617M,K618M,K632M,K633M was a gift from Elizabeth Wilson (Addgene plasmid # 115778 ; http://n2t.net/addgene:115778 ; RRID:Addgene_115778) -
For your References section:
A ligand-dependent bipartite nuclear targeting signal in the human androgen receptor. Requirement for the DNA-binding domain and modulation by NH2-terminal and carboxyl-terminal sequences. Zhou ZX, Sar M, Simental JA, Lane MV, Wilson EM. J Biol Chem. 1994 May 6;269(18):13115-23. PubMed 8175737