-
Plasmid#51087PurposeForebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterCaMKIIa 1.3 kbAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIA033
Plasmid#200420PurposethrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.DepositorArticleTypeEmpty backboneUseTagsbsPAmCherryExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIA034
Plasmid#200421PurposeamyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.DepositorArticleTypeEmpty backboneUseTagsbsdronPAExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-GluCl_cryst
Plasmid#31488DepositorInsertC. elegans glutamate-gated chloride channel alpha, crystallized construct
UseTags8x-HisExpressionInsectMutationPromoterAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIA028
Plasmid#200419PurposethrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloningDepositorArticleTypeEmpty backboneUseTagsPAmCherry1ExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFp10
Plasmid#170460PurposeEarlyBac insect cell expression systemDepositorInsertnone
UseTagsExpressionInsectMutationPromoterdHsp70Available sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato
Plasmid#51085PurposeNeuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV Retrograde and AAV1InsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterhuman Synapsin1Available sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS6-mPSD95a-TSt-HA
Plasmid#42237PurposeTimeSTAMP method for tagging and visualization of newly synthesized proteins. TimeSTAMPt cassette used for highly expressed proteins or proteins with faster ongoing synthesis ratesDepositorInsertPSD95
UseTagsTSt-HAExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
BDH2_FL_BioN
Plasmid#195870PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertBDH2 (BDH2 Human)
UsePfbd-bira, baculovirus expressionTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
p1177-X1-8
Plasmid#38150DepositorInsertCarboxypeptidase A
UseTagsHHHHHHRRExpressionInsectMutationPromoterpolyhedronAvailable sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIA027
Plasmid#200418PurposeamyE::(dronPA cm), for dronPA fusions. PALM-compatible integration vector for Bacillus subtilis. Includes dronPA photoswitchable FP with multi cloning sites (MCS) for fast and interchangeable cloning.DepositorArticleTypeEmpty backboneUseTagsdronPAExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-mNeonGreen
Plasmid#124102PurposeExpression of the fluorescent protein mNeonGreen in mammalian cellsDepositorInsertmNeonGreen
UseAAVTagsExpressionMammalianMutationCodon usage was optimized for Homo SapiensPromoterUbCAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-ER GFP
Plasmid#80069PurposeTo express and target GFP into the endoplasmic reticulum using lentivirusDepositorInsertER GFP (SEC61B Human)
UseLentiviralTagsGFP derived from Aequorea coerulescens on the NH2…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEZT-BM
Plasmid#74099PurposeMulti-purpose rapid titer bacmam expression vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseBacmam, baculovirusTagsEGFP following p10 promoterExpressionMammalianMutationPromoterCMV, p10Available sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-GFPplus
Plasmid#172718PurposeExpression of a fast folding GFP variant GFP plusDepositorInsertGFP plus
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
HDR-HaloPOLQ
Plasmid#211637Purposehalo donorDepositorInsertPuro-LoxHaloTag
UseTagsflagExpressionMammalianMutationPromoterSV40Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa-F
Plasmid#101193PurposeA fast switching Dronpa variantDepositorInsertffDronpa-F
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationK45I, V60A, F173VPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
-
pAAV2-CAGIG-WGA-mCherry
Plasmid#224119PurposeTo express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.DepositorInsertWGA-mCherry
UseAAVTagsWGA and mCherryExpressionMammalianMutationAll mutations related to the WGA in this construc…PromoterCAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only