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Plasmid#124864PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-KrasG12S/sgKras/Cre
Plasmid#99853PurposeExpresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.DepositorInsertKras (Kras Mouse)
UseAAV and Cre/LoxTagsExpressionMammalianMutationAvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-Chronos-GFP
Plasmid#122098PurposeAAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α (1.1 kb short version)Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-GFP
Plasmid#107709PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDR-sphys-3XHA
Plasmid#163690Purposeplasmid for homologous recombination-mediated insertion of triple HA tag into synaptophysin geneDepositorInserttriple HA tag insertion into endogenous mouse synaptophysin gene
UseAAVTagstriple HA tagExpressionMutationPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-E1.1-NRSE-RFP
Plasmid#22929DepositorInsertE1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Mac-GFP
Plasmid#58853PurposeAAV-mediated expression of Mac-GFP under the Syn promoterDepositorInsertMac-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-DIO-mAPX
Plasmid#79908PurposeAdeno-associated virus plasmid, Cre-dependent FLEX switch, mAPXDepositorInsertmAPX
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-OTp-hM3Dq-Myc
Plasmid#184753PurposeTo drive hM3D fused with Myc tag under the control of mouse oxytocin promoterDepositorInserthM3D-myc
UseAAVTagsMycExpressionMutationPromotermouse Oxytocin promoterAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-AAV2-hSyn-MerMAID6-Citrine
Plasmid#126521PurposeAnion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).DepositorInsertMerMAID6
UseAAVTagsCitrineExpressionMutationPromoterhuman synapsinAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-B7-EYFP
Plasmid#114110Purposefusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, slow burn
B7 hinge region
Enhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-Cas9
Plasmid#166601PurposeEncodes Cre-dependent Cas9 under control of the TREDepositorInsertCas9
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A
Plasmid#106185PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1InsertSF-Venus-iGluSnFR.S72A
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterhSynapsinAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1 Insulator ObLiGaRe/EPB71
Plasmid#90018PurposeDonor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locusDepositorInsertMCS flanked by insulator and inverted ZFN binding sites
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP
Plasmid#89061PurposeAAV-gRNA targeting the murine Ldlr geneDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Lyn-Y-GECO1
Plasmid#58200PurposeExpress the membrane targeted Y-GECO1 in neuronsDepositorInsertY-GECO1.0
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger1-TS-EYFP
Plasmid#127243PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterhSyn1Available sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-Venus-iGluSnFR.S72A
Plasmid#106191PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.S72A
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger1-TS-EYFP
Plasmid#127244PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCamKIIaAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP104-ChromeQ-mCherry
Plasmid#123125PurposeAAV-mediated expression of ChromeQ-mCherry under the GFAP promoter.DepositorInsertChromeQ-mCherry
UseAAVTagsmCherryExpressionMammalianMutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E9…PromoterGFAP104Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only