-
Plasmid#23068DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysine 5 to Glutamine H4 K5QPromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F47
Plasmid#23071DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysines 8 and 16 to Glutamines…PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F49
Plasmid#23073DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysines 8 and 16 to Arginine, …PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F51
Plasmid#23075DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysines 5 and 12 to Glutamine,…PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F52
Plasmid#23076DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H4 changed Lysines 5 and 12 to Arginines,…PromoterAvailable sinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F36
Plasmid#23069DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 14 to Glycine, H3 K14GPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F43
Plasmid#23070DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 14 to Arginine, H3 K14RPromoterAvailable sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
p031 pCS2 End-1 ORF
Plasmid#11030DepositorInsertEnd-1 (end-1 Nematode)
UseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEYmRD3A
Plasmid#127756PurposeResearch the effect of gene orientation of transcriptionDepositorInsertmSpinach,RFP Divergent Orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PPO-Venus
Plasmid#139505PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV8 and AAV9Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-PPO-Venus
Plasmid#139504PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV5Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2
Plasmid#52961PurposeReplaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NSAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSC226
Plasmid#216375PurposexhNup358-Nb2t (+3Cys) Nanobody productionDepositorInsertxhNup358-Nb2t (+3Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MDM2 WT
Plasmid#16233DepositorInsertMDM2 (MDM2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1187 VSVGmut
Plasmid#201913PurposeExpresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)DepositorInsertVSV-G (K47Q, R354A)
UseTagsExpressionMammalianMutationK47Q, R354APromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1362 scFv entry plasmid
Plasmid#201912PurposeEntry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)DepositorInsertStuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ZIM3-KRAB-dCas9
Plasmid#154472PurposeExpresses ZIM3 KRAB domain fused to the N terminus of dCas9DepositorInsertZIM3 (ZIM3 Synthetic)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV BAF170-Flag
Plasmid#19142DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TALEN-L
Plasmid#59025PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-L
UseAAV and TALENTags3xFlag and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TALEN-R
Plasmid#59026PurposeTALEN designed to target the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-TALEN-R
UseAAV and TALENTags3xFlag and NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only