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pAAV-FLEXfrt-SaCas9-U6-sgGrin2a

Plasmid

#124863

Purpose

Mutagenesis of Grin2a

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2a (Grin2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

Plasmid

#124859

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGria3

Plasmid

#124869

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

Plasmid

#124872

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

Plasmid

#124871

Purpose

Mutagenesis of Gabrg2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg2 (Gabrg2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E8-dTom-nlsdTom

Plasmid

#135643

Purpose

AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E8 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Insert

dTom-nlsdTom

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-KrasG12A/sgKras/Cre

Plasmid

#99849

Purpose

Expresses Cre-recombinase, a barcoded HDR template to introduce a G12A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

Depositor

Monte Winslow

Article

Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

Insert

Kras (Kras Mouse)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

Promoter

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-splitTVA950

Plasmid

#59329

Purpose

Expresses splitTVA950

Depositor

Ian Wickersham

Insert

splitTVA950

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA

Plasmid

#68346

Purpose

Used to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4

Depositor

Jannik Elverløv-Jakobsen

Article

Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

Inserts

3xgRNA;PTENA, p53B,SMAD4A
FlPO

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-GFPFANACfullWT

Plasmid

#126551

Purpose

AAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channel

Depositor

Rory McQuiston

Article

Schanuel et al Neuroscience. 2008 Aug 13;155(2):374-86.

Insert

eGFP N-terminal fused to FMRFamide gate sodium channel

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-APLP1ICD-IRES-hrGFP

Plasmid

#107546

Purpose

AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

APLP1ICD (APLP1 Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-REX-GECO0.9

Plasmid

#61249

Purpose

Expresses REX-GECO0.9 in neurons

Depositor

Robert Campbell

Article

Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

Insert

REX-GECO0.9

Use

AAV

Tags

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1: P60R, V61W, R6…

Promoter

hSyn

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-KrasG13V/sgKras/Cre

Plasmid

#99860

Purpose

Expresses Cre-recombinase, a barcoded HDR template to introduce a G13V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

Depositor

Monte Winslow

Article

Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

Insert

Kras (Kras Mouse)

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

Promoter

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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1184_pAAV-U6-ApoB-gRNA2-CB-EmGFP

Plasmid

#89062

Purpose

AAV-gRNA targeting the murine Apob gene

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Chicken beta actin with partial CMV enhancer

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA M4

Plasmid

#80937

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA M4 for Mstn cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-SEPT_Cdk2-T160E-siR

Plasmid

#73976

Purpose

HDR template to generate Cdk2-T160E-siR

Depositor

Steven Dowdy, Manuel Kaulich

Article

Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

Inserts

Cdk2-LHA-siR (CDK2 Human)
Cdk2-RHA-T160E (CDK2 Human)

Use

AAV

Tags

Expression

Mutation

T160 mutated to Glu and siRNA target site mutated

Promoter

Available since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV asyn S87D/S129A

Plasmid

#36071

Depositor

Hilal Lashuel, Patrick Aebischer

Insert

alpha-synuclein S87D/S129A (SNCA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

S87D/S129A

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pC129: pAAV.CMV-CasRx mCh

Plasmid

#203442

Purpose

Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

RfxCas13d-T2A-mCherry

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM148: pAAV.CMV-Cas13e-NT sgRNA

Plasmid

#203446

Purpose

Plasmid expressing active RfxCas13d with non-targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-non targeting (NT) sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sasgRNACMV- mCherry-WPREpA

Plasmid

#217015

Purpose

S. aureus sgRNAs backbone along with mCherry expression

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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