Addgene

We narrowed to 2,251 results for: PRK

Showing: 2041 - 2060 of 2251 results

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MAP2K2 gRNA (BRDN0001147931)

Plasmid

#77603

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ERK2-L4A-MEK1_fusion

Plasmid

#39197

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

L4A = L33A, L37A, L40A, L42A

Promoter

CMV

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-DnaJB1-PKAcat-mCherry

Plasmid

#181850

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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MAP2K1 gRNA (BRDN0001145794)

Plasmid

#76210

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K1 (MAP2K1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K1 gRNA (BRDN0001147078)

Plasmid

#76211

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K1 (MAP2K1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K1 gRNA (BRDN0001147218)

Plasmid

#76212

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K1 (MAP2K1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHRIG-Akt3

Plasmid

#53596

Purpose

lentiviral expression of constitutively active human Akt3 and EGFP

Depositor

Heng Zhao

Article

Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

Insert

cAkt3 (AKT3 Human)

Use

Lentiviral

Tags

HA and IRES-EGFP

Expression

Mammalian

Mutation

constitutively active (myristoylated version)

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only
MAPK3 gRNA (BRDN0001147876)

Plasmid

#75967

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK3 (MAPK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAP2K2 gRNA (BRDN0001147805)

Plasmid

#77600

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP2K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP2K2 (MAP2K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-MKK6(DD)-Puro

Plasmid

#86094

Purpose

Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

Depositor

Cindy Miranti

Article

Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

Insert

MKK6 (MAP2K6 Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Serine 207 and Threonine 211 both changed to Aspa…

Promoter

CMV/TetO

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-MEKDD

Plasmid

#31202

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S218D S222D

Promoter

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
pSLIK 3xHA-MKK6-EE neo

Plasmid

#47546

Purpose

Lentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MKK6-EE (MAP2K6 Human)

Use

Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

S207E, T211E

Promoter

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-EphB2-SV40-GFP (B2G)

Plasmid

#65442

Purpose

Expression of mouse EPHB2 and GFP in mammalian cells

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

EPHB2 (Ephb2 Mouse)

Use

Lentiviral

Tags

SV40-EGFP

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

UBQ

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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MAPK3 gRNA (BRDN0001147667)

Plasmid

#75968

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK3 (MAPK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK MEK5-DD-mRFP1 neo

Plasmid

#47548

Purpose

Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MEK5-DD-mRFP1 (MAP2K5 Human)

Use

Lentiviral

Tags

mRFP

Expression

Mammalian

Mutation

S311D, T315D

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
EIF2AK2 gRNA (BRDN0001148577)

Plasmid

#75638

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK2 (EIF2AK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AKT1 gRNA (BRDN0001146935)

Plasmid

#75500

Purpose

3rd generation lentiviral gRNA plasmid targeting human AKT1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AKT1 (AKT1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EIF2AK2 gRNA (BRDN0001146342)

Plasmid

#75637

Purpose

3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EIF2AK2 (EIF2AK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK3 gRNA (BRDN0001149014)

Plasmid

#75966

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK3 (MAPK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MEK1C222

Plasmid

#164638

Purpose

Bacterial expression plasmid for His6-MEK1C222

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Use

Tags

His6-tag

Expression

Bacterial

Mutation

G(-19)F/S222C/C277S/C376S

Promoter

T7

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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