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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39197 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)TG1
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameERK2-MEK1 Fusion
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SpeciesH. sapiens (human), R. norvegicus (rat)
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Insert Size (bp)2400
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MutationL4A = L33A, L37A, L40A, L42A
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GenBank IDM64300 NM_002755
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Entrez GeneMAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
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Entrez GeneMapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
rat ERK2fused to human MEK1 with a linker in between. L4A mutations are in the human MEK1 NES
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-myc-ERK2-L4A-MEK1_fusion was a gift from Melanie Cobb (Addgene plasmid # 39197 ; http://n2t.net/addgene:39197 ; RRID:Addgene_39197) -
For your References section:
A constitutively active and nuclear form of the MAP kinase ERK2 is sufficient for neurite outgrowth and cell transformation. Robinson MJ, Stippec SA, Goldsmith E, White MA, Cobb MH. Curr Biol. 1998 Oct 22;8(21):1141-50. 10.1016/S0960-9822(07)00485-X PubMed 9799732